LEGENDplex™ Human CD8/NK Panel (13-Plex) From BioLegend for Comprehensive Immune Effector Profiling

June 02, 2026

BlueskyReddit
George Washington University
Immunology
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

LEGENDplex™ Human CD8/NK Panel (13-plex) w/ VbP V02

Catalog Number:

741187

I used this kit to perform multiplex analysis of CAR T cell-derived cytokines and cytolytic proteins following pretreatment of tumor cells, revealing tumor-specific differences in inflammatory and granzyme secretion profiles associated with immune activation.

Experimental Design and Results Summary

Application

CAR T cell activation / immune effector profiling

Starting Material

CAR T cell supernatant

Tips

Careful optimization of effector-to-target ratios, coculture duration, and cytokine collection timepoints is important, as cytokine secretion kinetics can vary substantially across analytes and excessive tumor killing or low cell viability may affect detection sensitivity.

Results Summary

The LEGENDplex™ Human CD8/NK Panel enabled multiplex profiling of CAR T cell effector responses following PTT pretreatment and revealed tumor-specific differences in inflammatory cytokine and granzyme secretion patterns.

DOI or PMID #

N/A

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Summary

The Good

Simultaneous detection of multiple T cell- and NK-associated cytokines and cytolytic proteins from small sample volumes with robust sensitivity.

The Bad

Strong cytotoxic responses or low viable cell numbers can reduce measurable analyte levels and may require assay optimization.

The Bottom Line

A reliable multiplex platform for characterizing CAR T cell functional responses and immune activation dynamics in coculture systems.

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