High-throughput DNA Fragment Size Quantitation

April 27, 2026

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Research & Development
Research Scientist

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Company:

Agilent

Product Name:

HS NGS Fragment Kit (1-6000bp)

Catalog Number:

DNF-474-0500

The HS DNA NGS kit is designed for precise fragment analysis of DNA samples, making it well-suited for next-generation sequencing workflows. It enables accurate determination of DNA library fragment sizes, ensuring proper library preparation and quality control. In addition to sizing, the kit supports both qualitative and quantitative assessment of DNA fragments. This allows users to evaluate sample integrity, concentration, and distribution in a single workflow. Overall, it provides a reliable and efficient solution for comprehensive DNA fragment characterization.

Experimental Design and Results Summary

Application

DNA-Fragment analysis

Starting Material

DNA

Tips

It is a very sensitive kit and detects artifacts precisely

Results Summary

The electropherogram trace below shows DNA fragments mechanically sheared to approximately 350 base pairs (bp), analyzed using an HS NGS gel on the Fragment Analyzer 5300 system. The fragment distribution is tightly centered around the target size of 350 bp, indicating accurate and consistent shearing. The traces appear clean and well-defined, with no visible artifacts such as primer dimers or degradation products. Both the quality and quantity of the DNA fragments are within expected ranges, confirming successful sample preparation. Overall, the data demonstrate excellent fragment integrity and reliable performance of the analysis method.

DOI or PMID #

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Summary

The Good

The data quality and sensitivity

The Bad

Takes at least 1.5 hours for a whole plate (96 well format)

The Bottom Line

The DNA fragments are accurately sheared to the target size of ~350 bp, with a clean and well-defined electropherogram profile. Overall, the results confirm high-quality fragment distribution with no detectable artifacts and appropriate yield.

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