Unlocking NK Cell Function with BioLegend’s DNAM-1 Antibody

School of Medicine
Lambe Institute for Translational Research
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-human CD226 (DNAM-1) Antibody

Catalog Number:

338331

Clone Number:

11A8

This mouse anti-human antibody is specifically designed for optimal staining of the DNAM-1 activating receptor on human peripheral blood Natural Killer (NK) cells. DNAM-1 is essential for enhancing NK cell recognition and elimination of tumor and virus-infected cells. The antibody's high specificity ensures accurate binding, facilitating detailed analysis of NK cell activity and their role in immune responses.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human peripheral blood NK cells expanded with feeder cells

Primary Incubation

1:33 dilution, for 20 min at room temperature

Blocking Agent

PBS + 10% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek NL2000

Results Summary

This mouse anti-human antibody offers excellent staining of the activating receptor DNAM-1 on the surface of human peripheral blood NK cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Has good resolution.

The Bad

None.

The Bottom Line

The antibody delivers excellent resolution, allowing for clear and distinct separation between positive and negative populations. This high level of sensitivity and specificity ensures reliable detection, even when target expression is low or variable. The staining is consistent and reproducible across experiments, making data interpretation straightforward and robust. Overall, its strong performance and dependable results make it a highly recommended choice for accurate and confident analysis.

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