Invitrogen
Galectin 3 Monoclonal Antibody
14-5301-82
M3/38
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I utilize Galectin-3 antibody staining to assess lysosomal membrane integrity in my studies. I observe the recruitment of Gal3 to damaged lysosomes, which allows me to evaluate the lysosomal stress responses induced by LLoMe.
Immunofluorescence
Methanol fixed cells
1:100
Sapnin, Donkey Serum, BSA
Anti-Rat AF594
None
Confocal microscopy
In my imaging, the red signal (Galectin-3) appears as puncta that localize to sites of lysosomal damage.The green signal (LAMP1) marks the lysosomes, and the overlap confirms Gal3 recruitment to compromised lysosomal membranes.
N/A
Specific signal.
None.
This is a good antibody because it specifically detects Galectin-3, producing clear puncta that make lysosomal damage easy to visualize alongside LAMP1.