This EGR1 Antibody is an Excellent Choice for Accurately Detecting EGR1 in Pancreatic Islets Through IHC

March 07, 2025

BlueskyReddit
Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

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Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

EGR1 (44D5) Rabbit mAb

Catalog Number:

#4154

Clone Number:

44D5

Jun B was studied in mouse pancreatic islets. The results indicated that EGR1 can be detected in the cell nucleus.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse pancreatic tissue

Primary Incubation

1:400 overnight at 4 degrees

Blocking Agent

5% horse serum 1 hour

Secondary Incubation

Universal horse anti-rabbit secondary antibody

Tertiary Incubation

1 hour at room temperature

Detection

DAB staining

Results Summary

The results the antibody specific detected EGR1 (red arrow) in cell nucleus, indicating hyperproliferative property.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Efficient for IHC.

The Bad

N/A

The Bottom Line

This antibody is good to detect pancreatic islet by IHC.

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