Thermo Fisher Scientific
VCAM-1 Monoclonal Antibody (1.4C3)
MA5-11447
1.4C3
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The VCAM-1 monoclonal antibody was obtained from ThermoFisher Scientific to immunostain VCAM-1 proteins in human endothelial cells. Human primary endothelial cells were grown to full confluence, then growth was arrested and the cells were fixed in paraformaldehyde. Following this, the cells were immunofluorescence-stained with the VCAM-1 monoclonal antibody. The immunostained cells were observed under a fluorescent microscope, and images were captured. Cells stained with control IgG served as a negative control. The choice of antibody from ThermoFisher Scientific was based on information provided in their antibody flyer.
Immunofluorescence
Human endothelial cells
1:200 dilution, overnight at 4 degree Celsius.
5% BSA
1:500 dilution, Goat anti-mouse IgG Alexa Fluor 568, 2 hours at RT
None
Fluorescent microscopy
The VCAM-1 antibody from Thermo Fisher Scientific was highly specific for human VCAM-1 and successfully immuno-stained VCAM-1 proteins.
N/A
Its specificity.
The VCAM-1 monoclonal antibody from Thermo Fisher Scientific was highly specific to human VCAM1 protein and I recommend it.