Excellent Marker From Merck Millipore for Astrocytes in the Mouse Brain

Neuroscience
Wisconsin University
Postdoctoral Researcher

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Quality of Results

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Company:

AB5535

Product Name:

Anti-SOX9 Antibody

Catalog Number:

AB5535

Clone Number:

N/A

Info: Supplier Page 

Browse Similar Products: Anti-SOX9 Antibody Products

We utilize this antibody to investigate neuroinflammation in animal models of neurodegenerative diseases. The specific objective of the experiment was to quantify astrocytes. Immunolabeling using this antibody highlights SOX9-positive mouse astrocytes.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:500 at 4 degrees celsius overnight

Blocking Agent

5% normal donkey serum

Secondary Incubation

Alexa fluor anti-rabbit IgG at 1:500 for overnight at room temp

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

This Sox9 antibody worked well to stain mouse astrocytes in the brain by immunofluorescence. It is a highly specific antibody so that we see little background with immunostaining on tissue section.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The staining is strong, with no non-specific binding observed.

The Bad

It is a polyclonal antibody.

The Bottom Line

Highly recommended for astrocyte staining in the central nervous system.

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