MHCII Anti-Mouse Antibody With Bright Signal

AG 61
Leibniz Institute für Virologie
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-mouse I-A/I-E Antibody

Catalog Number:

107617

Clone Number:

M5/114.15.2

The antibody was used to stain cells derived from mouse lungs. It produced a strong signal.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse cells

Primary Incubation

1/300 in PBS/FCS, 25 minutes at RT

Blocking Agent

CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Very good detection of MHCII positive cells. The signal is bright and it allows an easy distinction of positive and negative mouse cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and reliable signal.

The Bad

None.

The Bottom Line

Highly recommended antibody for mouse.

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