Fig 1: ASCs and immune cells comprise a dynamic cellular niche.(A, top) Representative images of fixed frozen mouse cold-exposed iBAT. Tissue was stained with smFISH probes Dcn (green), H2-Ab1 (red), and Top2a (pink). Nuclei were counterstained with DAPI. Images on the right are a magnified view of the boxed regions on the left. Scale bars, 40 µm and 5 µm. (A, bottom) Quantification of cell types within 20 µm around either an H2-Ab1+ cell or a Dcn+ Top2a+ cell (n=3 animals; >100 cells/mouse; mean ± SD). (B) Representative images of fixed frozen mouse cold-exposed iBAT. Tissue was stained with smFISH probes Dcn (green), H2-Ab1 (red), and Nnat (pink). Nuclei were counterstained with DAPI. Images on the right are a magnified view of the boxed regions on the left. Scale bars, 40 µm and 5 µm. (B, bottom) Quantification of cell types within 20 µm around either an H2-Ab1+ cell or a Nnat+ cell (n=3 mice; >100 cells/mouse; mean ± SD). See also Figure 8—source data 1, Figure 8—source data 2. Figure 8—source data 1.Quantification of the nearest neighbor (20 µM) to either a H2-Ab1+, Dcn+, or Dcn+ Top2a+ cell in the iBAT of cold-exposed mice. Figure 8—source data 2.Quantification of the nearest neighbor (20 µM) to either a H2-Ab1+, Dcn+, or Nnat+ cell in the iBAT of cold-exposed mice.