Perfect Marker for Degranulation From BioLegend

August 30, 2024

BlueskyReddit
Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human CD107a (LAMP-1) Antibody

Catalog Number:

328608

Clone Number:

H4A3

We generate tumour-specific T cells and test their efficacy during a co-culture with tumour cell lines.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human primary T cells

Primary Incubation

CD107a and protein transport inhibitor

Blocking Agent

Human TruStain Fc block, 5uL diluted in 95uL FACS buffer (5% FBS in DPBS), incubated before adding CD107a

Secondary Incubation

Desired antibody and fixative (as needed)

Tertiary Incubation

N/A

Detection

Flow cytometry in a BD Celesta or Cytek Aurora cytometer

Results Summary

This antibody helped us find cytotoxic T cells in our T cell product, helping us characterize the properties and function of our product.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and fairly sensitive.

The Bad

Difficult to optimize CD107a marker.

The Bottom Line

Great signal once you get it to work!

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