Ideal Antibody From Cell Signaling Technology For Western Blot With a Clear Signal

July 27, 2024

BlueskyReddit
Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

MDM2 (D1V2Z) Rabbit mAb

Catalog Number:

86934S

Clone Number:

#86934

To test the effect of IPS neuron differentiation on MDM2 protein level. The results showed that the differentiation of IPS to neuron cells could change the MDM2 expression.

Experimental Design and Results Summary

Applications

Western blot

Sample

IPSc sample

Primary Incubation

1:1000 overnight at 4 degrees

Blocking Agent

5% milk in TBS

Secondary Incubation

1:3000, 1 hour at room temperature.

Tertiary Incubation

N/A

Detection

ECL

Results Summary

To test the effect of IPS neurons differentiation on MDM2 protein level. The results showed that differentiation of IPS to neuron cell could change the MDM2 expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody for Western Blot.

The Bad

N/A

The Bottom Line

This antibody has very good signaling in IPS cell.

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