Western Blot on A549 With ATM (D2E2) Rabbit mAb From Cell Signaling

February 09, 2024

BlueskyReddit
Northbridge Biomedical Research Institute

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Company:

Cell Signaling Technology

Product Name:

ATM (D2E2) Rabbit mAb

Catalog Number:

2873S

Clone Number:

This antibody (#2873S) was used on A549 stimulated with TGF-b for 24 hours. The primary antibody was diluted 1:1000 in 5%BSA in 1x TBST.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A549

Primary Incubation

1:1000

Blocking Agent

5% BSA in TBST

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

This antibody (#2873S) was used on A549 stimulated with TGF-b for 24 hours. The primary antibody was diluted 1:1000 in 5%BSA in 1x TBST.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The band was visible but the signal was weak.

The Bad

Band has some background.

The Bottom Line

Overall it is recommended.

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