Abcam
Anti VCAM1 antibody [EPR5047]
ab134047
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VCAM1 is a cell adhesion molecule that regulates inflammation-associated vascular adhesion. Our goal was to detect VCAM1 in the brains of rats.
Immunofluorescence
Rat brain
1:100
Normal Goat Serum diluted in 0.1% BSA
AlexaFluor 488 goat anti-rabbit
None
Confocal - fluorescence
Upon several optimization steps, we were able to detect VCAM-1 in the brain. Please note that this rat model was not of inflammation, which might explain the low to medium levels of VCAM-1. Antigen retrieval: TRIS-EDTA (pH9)0.1% Triton X-100.
N/A
Works well in rat samples.
It might need optimization.
We believe the antibody works, but the intensity of the signal is dependent on an inflammatory state.