CellTiter 96® AQueous One Solution Cell Proliferation Assay From Promega With Optimization Works Perfectly

November 04, 2023

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Promega

Product Name:

CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Catalog Number:

G3582

Clone Number:

N/A

I used this product to determine the cytotoxicity of my T cells against my tumor cells. This reagent enables me to do so without the need to label my target cell line. With optimization, I found this reagent greatly simplified my co-culture workflow.

Experimental Design and Results Summary

Applications

Viability assay

Sample

Tumor cell line

Primary Incubation

1 to 4 hours in the 37C incubator (in complete darkness)

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Absorbance by spectrophotometer

Results Summary

Using this reagent allowed me to determine the amount of viable tumor cells remained following co-culture with my T cells. The reagent was able to produce signal that made clear the amount of live tumor cells were in my samples.This took some optimization as I realized the absorbance wavelength of formazan overlaps with phenol red, so I had to use phenol red free media during the incubation with the reagent.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very simple workflow.

The Bad

Very sensitive so replicates are important as well as good pipetting technique.

The Bottom Line

This reagent allows a very simple workflow for evaluating cytotoxicity. With optimization, I am able to use this reagent for my needs.

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