Prominent Antibody From BioLegend

October 23, 2023

BlueskyReddit
Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-human CD8a Antibody

Catalog Number:

301046

Clone Number:

N/A

We are an immunotherapy-focused group interested in CD8 T-cells and their TCRs. We routinely use T-cell-based assays for our experimental purposes, so this antibody is part of our panel.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

PBMCs

Primary Incubation

1:100, 15min RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

Using the BV705-aCD8 antibody, a clear separation of the CD8 T-cells is observed, when PBMCS were stained with it.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, and works wells.

The Bad

None.

The Bottom Line

The antibody works well and is reliable between different batches. BV705 might not be the brightest fluorochrome, but we have used other conjugates of the same antibody and they are also performing really well.

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