Cell Signaling Technology
Phospho-mTOR (Ser2448) Ab
5536S
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The cell line was infected with mock or test viral particles (10 moi) and after 36 hours of incubation in complete media the cell extracts were prepared, and proteins were resolved in SDS-PAGE and immunoblotted with Phospho-mTOR (Ser2448) antibody. The antibody was purchased from Cell Signaling Technologies by its merit and referral from published articles and reviews.
Western blot
Total extract from virus infected and non-infected cells
1:1000 in BSA (5%), overnight at 4 degrees celsius
BSA (5%), 2h at RT
Goat-anti rabbit IgG-HRP (1:4000), 2h at RT
None
ECL
The antibody showed high specificity to mTOR (Ser2448) as only one band detected at expected molecular mass. The antibodies never cross reacted with non-specific proteins. There was a change of mTOR (Ser2448) levels in mock and test virus infected group.
N/A
Its specificity to detect mTOR (Ser2448).
None.
I highly recommend this antibody.