Thermo Fisher
Blasticidin S HCl (10 mg/mL)
A1113903
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Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking the hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian cells and bacterial cells.
Cell culture
Cultured mammalian cells
I use the recommended dilution
The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.
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Great price and works well.
Can't think of anything bad.
I use this a lot and have been using this same brand for a long time. I trust it and it works really well.