Good Staining for GATA3 With Antibody From BD Biosciences

Wayne State University

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Company:

BD Biosciences

Product Name:

BD Horizon™ BUV395 Mouse Anti-GATA3

Catalog Number:

565448

Clone Number:

L50-823

Info: Supplier Page

Browse Similar Products: Anti-GATA3 Antibody Products

Gata3 staining with this antibody is good. Separation of the positive population could be a bit better, but staining in T cells is difficult.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Single cell suspensions from mouse intestine show a positive population when gated on T cells. However, the separation is not the best.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Shows a positive population.

The Bad

Not a great separation in T cells.

The Bottom Line

T cell GATA3 staining can be a bit difficult, but this antibody does show a population of GATA3 positive cells.

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