ThermoFisher
CD8a Monoclonal Antibody (53-6.7), PE-Cyanine7, eBioscience™
25-0081-82
53-6.7
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When analyzed by flow cytometry, there is some variability in the staining, which produces a smear. Decent, but not the best.
Flow cytometry
Mouse intestine
1:200 for 15 min at 4 degrees C
Fc block
N/A
Fortessa LSRII
Mouse intestine cells stained with this antibody shows a smear in the T cell compartment, although this could be due to the sample.
Clear positive population.
Shows up as smear.
The antibody works, but is not the best. Although, these results could be due to the sample and not the antibody.