Foolproof Nuclear Stain From Thermo Fisher

University of Texas Medical Branch at Galveston
OBGYN
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

NucBlue™ Live Cell Stain ReadyProbes ™ reagent

Catalog Number:

R37605

We do immunofluorescence experiments to visualize cells seeded on chips. This nuclear stain has never failed us in visualizing cell nuclei.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

Live cells

Tips

Only mix two drops of reagent per mL of solution.

Results Summary

We would always get a crisp blue signal for our cell nuclei. The reagent only needs 5 min of incubation to set the stain. The stained cells last >30 days on 4 degrees.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Very cheap and reliable.

The Bad

N/A

The Bottom Line

This stain is the best whenever you are interested in doing immunofluorescence, perhaps better than a Hoechst stain. It is also cheap and comes in a very handy dropper-like container.

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