Bradford Assay Kits

Bradford assay kits include the essential reagents for quantifying the abundance or concentration of total proteins in biological samples. Also known as the Coomassie protein assay, the Bradford protein assay is a well-known standard assay using the Coomassie Brilliant Blue G-250 dye. The dye strongly binds proteins to form a blue protein-dye complex, which enables colorimetric spectroscopic detection at 595 nm. Bradford assay kits generally have a protein detection range of 125–1,500 µg/mL. It is a rapid, straightforward assay that is resistant to interference from small quantities of salts, carbohydrates, and reducing agents. However, the presence of detergents such as SDS and Triton X-100 can interfere with the color of the assay. When choosing an assay kit, consider important specifications such as the assay detection range, recommended sample types, and the components included within the kit. Visit the supplier page for more details.