SNAIL/SLUG Antibody From Abcam Works Fine

Pathology
Sao Paulo State University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Abcam

Product Name:

Anti-SNAIL + SLUG antibody

Catalog Number:

ab180714

Clone Number:

N/A

Currently working with pregnancy-related cells and doing protein analysis of different markers upon different treatments (i.e., bacteria infection and inflammation).

Experimental Design and Results Summary

Applications

Western blot

Sample

Human amnion epithelial cells

Primary Incubation

1:500 dilution, overnight at 4 degrees

Blocking Agent

5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)

Secondary Incubation

1:10000, 2 hours, RT

Tertiary Incubation

N/A

Detection

FACSCalibur (Becton Dickinson), software CellQuest version 4

Results Summary

The results do not look as good as expected. The bands are not very clear and there are background. We also tried 1:2000 and 1:1000, but the best results are from 1:500 dilution.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to work.

The Bad

Not good bands.

The Bottom Line

This antibody is very easy to work with, but has background.

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