Potent Staining for Mitochondria With Dye From Invitrogen

September 09, 2023

BlueskyReddit
Robert Bosch Gesellschaft für medizinische Forschung mbH
IKP/RBCT
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific, Invitrogen

Product Name:

MitoTracker™ Red CMXRos Dye

Catalog Number:

M46752

Info: Supplier Page 

The Mitotracker Red CMXRos Dye from Invitrogen is a very high-yield dye for staining of Mitochondria in vitro. The Dye is quite stable and bright and can be used in fixed or viable cells without problems.

Experimental Design and Results Summary

Application

IF staining, flow cytometry

Starting Material

viable cells e.g. HEK293, MCF-7

Tips

Start at a very low concentration (100 nM is usually enough), make sure to wash out unbound dye with fresh medium, possibly fix cells afterwards.

Results Summary

The dye is even at low concentrations very bright (100 nM, 30 min incubation). In fixed cells the dye remains stable and is perfectly suitable for IF stainings (excitation in red channel e.g. 552 nm laser). However, since its so bright and high-yield it is sometimes not 100 % specific. Optimization with different concentrations might be necessary. Quality of results depends on the sample.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

High-yield, bright and easy protocol.

The Bad

Specificity and optimization is needed.

The Bottom Line

If you ever wanted to stain mitochondria in a quick and easy manner I totally recommend the Mitotracker Red staining. In contrast to Mitotracker green it can be combined with fixation and usually gives you bright signals.

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