Good PE-Cy7 Anti-Mouse KLRG1 Ab From Invitrogen

Department of Fundamental Oncology
University of Lausanne
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

KLRG1 Monoclonal Antibody (2F1), PE-Cyanine7, eBioscience

Catalog Number:

25-5893-82

Clone Number:

2F1

To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mice were stained with: PerCP-Cy5.5 anti-CD4 Ab and PE-Cy7 anti-KLRG1 Ab in the presence of Zombie NIR in 100 µL PBS at 4C for 30 min.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from C57BL/6 mouse

Primary Incubation

See description

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRFortessa

Results Summary

To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab and PE-Cy7 anti-KLRG1 Ab in the presence of Zombie NIR in 100 μL PBS at 4C for 30 min.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great resolution.

The Bad

N/A

The Bottom Line

According to the titration, 1:100 dilution has the best SI.

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