IL-12 Antibody AF-219-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-12 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $569.00Supplier Page View Company Product Page
Catalog NumberAF-219-NA
NCBI Gene AliasesCLMF, IL-12A, NFSK, NKSF, NKSF2, NKSF1, CLMF2, P35, IMD29, IMD28, IL-12B
ApplicationsAffinity Chromatography,Bioassay,Immunohistochemistry,Western Blot,Neutralization,Immunocytochemistry
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
HostGoat
TargetIL-12
Quantity100 ug (also 25 ug)
ReactivityHuman
NCBI Full Gene Nameinterleukin 12B, interleukin 12A
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 heterodimer, Arg23-Ser219 of p35; Ile23-Ser328 of p40, Accession # P29459 (p35) & P29460 (p40)
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IL-12 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) ABT-263, a BCL-2 inhibitor, selectively eliminates latently HIV-1-infected cells without viral reactivationPLoS OneMay 20, 2025Jeong Eun Kang, Hyun Wook Seo, Dong-Eun Kim, Young Hyun Shin, Songmee Bae, Cheol-Hee Yooncultured with 10 µg/ml IL-4 antibody (R&D system, cat# MAB204), 20 µg/ml IL-12 antibody (R&D system, cat# AF-219-NA), 4 µg/ml recombinant TGF-β1 (R&D system, cat# 7754-BH-025), and 30 U/1 × 106 cells of human IL-2 (Roche,

(2) Chronic stimulation desensitizes β2-adrenergic receptor responses in natural killer cellsEuropean journal of immunologyDecember 1, 2024Jürgens M, Claus M, Wingert S, Niemann JA, Picard LK, Hennes E, Haasler I, Hellwig B, Overbeck N, Reinders J, et al.

(3) A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancerCell death & diseaseMay 1, 2023Zhao MN, Zhang LF, Sun Z, Qiao LH, Yang T, Ren YZ, Zhang XZ, Wu L, Qian WL, Guo QM, et al.

(4) Fungal sensing by dectin-1 directs the non-pathogenic polarization of T(H)17 cells through balanced type I IFN responses in human DCsNature immunologyDecember 1, 2022Gringhuis SI, Kaptein TM, Remmerswaal EBM, Drewniak A, Wevers BA, Theelen B, D'Haens GRAM, Boekhout T, Geijtenbeek TBH.neutralizing antibodies (5 μg ml−1) to IL-1β (AF-201-NA, R&D Systems), IL-23 (AF1716, R&D Systems), IL-12 (AF-219-NA, R&D Systems), IL-27 (AF2526, R&D Systems) or TGF-βRII (AF-241-NA, R&D Systems) or normal goat IgG (AB-108-C;

(5) Inhibition of G-protein βγ signaling enhances T cell receptor-stimulated interleukin 2 transcription in CD4+ T helper cellsPloS oneJanuary 1, 2015Yost EA, Hynes TR, Hartle CM, Ott BJ, Berlot CH.

(6) The p40 subunit of interleukin (IL)-12 promotes stabilization and export of the p35 subunit: implications for improved IL-12 cytokine productionThe Journal of biological chemistryMarch 1, 2013Jalah R, Rosati M, Ganneru B, Pilkington GR, Valentin A, Kulkarni V, Bergamaschi C, Chowdhury B, Zhang GM, Beach RK, et al.secondary antibody (catalog no. 401504, Calbiochem). The polyclonal human IL-12p70 antibody (catalog no. AF219-NA; cross-reactive with rhesus IL-12p70), and the polyclonal murine IL-12p70 antibody (catalog no. AF419-NA,

(7) Imidazoquinoline Acts as Immune Adjuvant for Functional Alteration of Thymic Stromal Lymphopoietin-Mediated Allergic T Cell ResponseThe Journal of ImmunologyOctober 15, 2008Yoshitaro Torii, Tomoki Ito, Ryuichi Amakawa, Hiroyuki Sugimoto, Hideki Amuro, Tsutomu Tanijiri, Yuichi Katashiba, Makoto Ogata, Takashi Yokoi, Shirou Fukuharaused the following reagents for culture conditions: anti-OX40L mAb (ik-5: 50 ␮g/ml), anti-IL-12 Ab (AF219-NA, R&D Systems: 1 ␮g/ml), and recombinant soluble OX40L (200 ng/ml; R&D Systems). Mouse IgG2a and Goat

(8) Mycobacterium bovis Bacillus Calmette-Guérin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cellsInternational immunologyOctober 1, 2008Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, et al.

(9) Upregulated IL-23 and IL-17 in Behçet patients with active uveitisInvestigative ophthalmology & visual scienceJuly 1, 2008Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A.

(10) Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cellsEuropean journal of immunologyApril 1, 2008Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S.

(11) Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytesJournal of immunology (Baltimore, Md. : 1950)May 1, 2006Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC.

(12) Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytesJournal of immunology (Baltimore, Md. : 1950)May 1, 2006Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC.

(13) Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccinationVaccineMarch 20, 2006Nagarajan S, Selvaraj P.

(14) Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytesJournal of virologyMarch 1, 2006Koelle DM, Huang J, Hensel MT, McClurkan CL.

(15) TSLP-activated dendritic cells induce an inflammatory T helper type 2 cell response through OX40 ligandThe Journal of experimental medicineNovember 7, 2005Ito T, Wang YH, Duramad O, Hori T, Delespesse GJ, Watanabe N, Qin FX, Yao Z, Cao W, Liu YJ.μg/ml anti-OX40L mAb (ik-5), 10 μg/ml anti–IL-4 mAb (MP4-25D2; BD Biosciences), 1 μg/ml anti–IL-12 Ab (AF-219-NA; R&D Systems), 10 μg/ml anti-CD40L mAb (LL2), 10 ng/ml recombinant human IL-12 (R&D Systems), and 25

(16) Effect of piceatannol in human monocyte-derived dendritic cells in vitroJournal of pharmaceutical sciencesMay 1, 2005Takei M, Umeyama A, Arihara S, Matsumoto H.

(17) Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanismJournal of immunology (Baltimore, Md. : 1950)February 15, 2005Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D.

(18) Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanismJournal of immunology (Baltimore, Md. : 1950)February 15, 2005Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D.

(19) Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10Journal of immunology (Baltimore, Md. : 1950)May 1, 2004Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA.

(20) APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNAJournal of immunology (Baltimore, Md. : 1950)January 1, 2004Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ.

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(1)

8/12/2023Good, But Pricey Antibody From R&D Systems Michael LehrkeWe performed in vitro T helper polarization assays using human CD4+ T cells. We utilized this anti-human IL-12 antibody, along with an anti-human IFNg antibody, to prevent unwanted Th1 polarization in... Read Review

IL-12 Antibody

IL-12 Antibody

IL-12 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (24)

Reviews

IL-12 Antibody from R&D Systems, a Bio-Techne Brand

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