Ki-67 (D2H10) Rabbit mAb 9027S from Cell Signaling Technology

Product Specs
ItemKi-67 (D2H10) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number9027S
Quantity100 µl
ApplicationsIHC-p
ReactivityH, Mk
ClonalityMonoclonal
TargetKI-67
HostRabbit
NCBI Full Gene Namemarker of proliferation Ki-67
NCBI Gene AliasesKIA, MIB-, MIB-1, PPP1R105
Research AreaCell Cycle / Checkpoint Control
IsotypeIgG
CloneD2H10

Questions and Answers

Can this antibody be used for studying cell proliferation in cancer research?

Yes, the Ki-67 (D2H10) Rabbit mAb has been utilized in various studies to assess cell proliferation in cancer research. For instance, it was employed in immunohistochemistry to evaluate tumor growth and cellular proliferation in colorectal cancer models, demonstrating its relevance in understanding tumor dynamics ().

Is this antibody effective for immunohistochemistry in human tissues?

The Ki-67 (D2H10) Rabbit mAb has been shown to work effectively in immunohistochemistry on human tissues. It has been cited in multiple studies, including those analyzing tumor samples from breast cancer and glioblastoma, confirming its utility in detecting Ki-67 protein levels in paraffin-embedded sections (, ).

What is the recommended dilution for using this antibody in immunohistochemistry?

The recommended dilution for the Ki-67 (D2H10) Rabbit mAb in immunohistochemistry is typically 1:200, as indicated in various studies that utilized this antibody for staining tumor tissues ().

Does this antibody have any specific applications in studying drug resistance in cancer?

Yes, the Ki-67 antibody has been referenced in studies exploring drug resistance mechanisms in cancer. For example, it was used to evaluate cellular senescence and proliferation in HER2-positive breast cancer models, contributing to the understanding of lapatinib resistance ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) JIN-A02, a Mutant-Selective Fourth-Generation EGFR Inhibitor, Overcomes C797S-Mediated Resistance and Demonstrates Intracranial Activity in NSCLCClinical Cancer ResearchMay 1, 2026Eun Ji Lee, Ji Ae Ko, Min-je Kim, Jae Seok Cho, Ji-Youn Han, Sang We Kim, Ki Hyeong Lee, Byoung Yong Shim, Jong-Mu Sun, Misako Nagasaka, et al.normal goat serum (Dako), after which slides were incubated for 1 hour with an anti-Ki67 antibody (no. 9027, Cell Signaling Technology, RRID: AB_2636984). Slides were washed with 50 mmol/L Tris-Cl (pH 7.4) and

(2) A multi-omics approach to identify the impact of miR-411ed on NSCLC TKI resistancebioRxivApril 3, 2026Daniel Del Valle Morales, Giulia Romano, Michela Saviana, Patrick Nana-Sinkam, Giovanni Nigita, Mario Acunzoand Cleaved Caspase-3 for 45 minutes. Antibodies used were: Ki-67 (1:3000, Cell Signaling Technology #9027), Cleaved Caspase-3 (1:500, Cell Signaling Technology #9664), and Transferrin Receptor (1:6000, ThermoFisher

(3) Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling AxisInternational Journal of Molecular SciencesMarch 31, 2026Grace L. Wong, Munazza S. Khan, Sara Manore, Shivani Bindal, Ravi Singh, Hui-Wen Loconducted as previously described [78,79]. IHC antibodies included Ki-67 (Cell Signaling Technology; cat. #9027S), GFAP (Abcam; cat. # 68428), ZNF24 (Proteintech, Rosemont, IL, USA; cat. #11219-1-AP), and PTEN (Cell

(4) Novel Triazoloquinoxaline-Based Tubulin Polymerization Inhibitor Induces Necroptosis and Significantly Inhibits Metastatic Melanoma Tumor GrowthJournal of Medicinal ChemistryMarch 26, 2026Christopher J. Clark, Shelby N. Waddell, Garrett E. Tessmer, Carl B. Womack, Yang Xie, Brittney D. Williams, Meirola A. Endraws, Cole T. Huddleston, Keiluhn D. Pulis, Joshua Thammathong, et al.primary antibody overnight at 4 °C in humid chambers: rabbit anti-Ki67 (Cell Signaling Technology #9027 (human reactivity), 1:400 and #12202 (mouse reactivity), 1:400); rabbit anticleaved-caspase 3 (CST #9661,

(5) CS2164 combined with radiation suppressed tumor growth via γ-H2AX persistence and ROS accumulationBMC CancerMarch 18, 2026Shun-li Peng, Yu-ling Zhou, Xuexia Liang, Ren-wei Jiang, Shuai Yang, Qiao-dan Liu, Xiao-mou Penganti-CD31 monoclonal antibody (1:800 dilution, #3528) and Ki-67 monoclonal antibody (1:400 dilution, #9027) were purchased from Cell Signaling Technology. The horseradish peroxidase-conjugated secondary antibodies

(6) Carnosic Acid Activates the STING/IRF3 Pathway to Induce Nitric Oxide-Mediated Apoptosis in Osteosarcoma CellsAntioxidants (Basel)March 16, 2026Weixiong Guo, Lanlan Yin, Qiang Wu, Jiaqi ChuSections were then incubated overnight at 4 °C with primary antibodies targeting Ki67 (1:200, CST, Cat# 9027), STING (1:200, CST, Cat# 13647), p-TBK1 (1:200, CST, Cat# 5483), and p-IRF-3 (1:200, CST, Cat# 4947).

(7) Dual Aptamers‐Based SETDB1 PROTACs as Effective Anti‐Tumor Strategies for Breast CancerAdvanced ScienceMarch 1, 2026Yanxuan Guo, Yingge Lv, Shuyu Huang, Chang Liu, Yan Ouyang, Bei Lan, Chenghao Xuananti‐ubiquitin (ab134953), and anti‐MDM2 (ab259265) from Abcam Ltd. (Cambridge, MA, USA); anti‐Ki67 (9027) from Cell Signaling Technology Inc. (Danvers, MA, USA); anti‐β‐actin (AC026) and anti‐NCL (A5904) from

(8) 3D “emboli” culture models epithelial breast cancer cell oxidative mitochondrial metabolism with relevance for lung metastasisCancer Research CommunicationsMarch 1, 2026Kuppusamy Balamurugan, Melissa R. Mikolaj, Jonathan M. Weiss, Ronald J. Holewinski, Yu Fan, Shiba Prasad. Dash, Xia Xu, Lois McKennett, Christopher W. Dell, Duncan Donohue, et al.antibodies for ID1 at 1:50 (Cell Signaling Technology #23369), Ki67 at 1:200 (Cell Signaling Technology #9027), or isotype control rabbit monoclonal IgG (Cell Signaling Technology # 3900). All experiments were

(9) B7-H3-mediated cis-inhibition of EGFR by a tumor-selective bispecific antibody enhances anti-tumor efficacy and minimizes toxicitiesNature CommunicationsFebruary 24, 2026Jian Guan, Tiongsun Chia, Bin Li, Tianyu Zhu, Zhengguang Liao, Junjie Deng, Fenggen Fu, Weiwei Wu, Chengtao Liu, Ya Liu, et al.S6 (CST, 2217S), pS6 (CST, 2211S), GAPDH (CST, 2218), Pan CK (Gene Tech, GM351514), and Ki67 (CST, 9027S). Affinity and epitope binning measurement The binding affinity and kinetics were assessed using biolayer

(10) Spermine Oxidase Serves as a Key Functional Node in Microbial Dysbiosis-Induced Breast CarcinogenesisCancer ResFebruary 16, 2026Deeptashree Nandi, Sheetal Parida, Deepak Verma, Jackson R. Foley, Tracy Murray Stewart, Preethi Korangath, Sowjanya Thatikonda, Sumit Siddharth, Qitong Wu, Mingyang Yi, et al.(MA532192) from Thermo Scientific; SMOX (15052-1-AP, Proteintech for immunoblotting); γ-H2AX (9818S), Ki-67 (9027S), E-cadherin (3195S), CD45 (70257S), CD3 (99940S), CD8 (98941S), CD11b (93169S), F4/80 (70076S) and

(11) MBD3 increased expression by BRD4 and facilitated castration-resistant prostate cancer cell proliferation by inhibiting PTENDiscover OncologyFebruary 9, 2026Liangming Pan, Jianliang Shen, Zhi Li, Guilin Xie, Cong Chen, Xi ChenCompany (Wuhan, China). Cleaved caspase 3 antibody (Catalog No.9661 S), and Ki67 antibody (Catalog No.9027 S) were purchased from Cell Signaling Technology. HRP AfniPure Goat Anti-Rabbit IgG (Catalog No. A0216)

(12) Histone H4K8 lactylation promotes glioblastoma progression by inducing NUPR1-mediated autophagosome‒lysosome fusionTheranosticsFebruary 4, 2026Jiangli Zhao, Xuchen Liu, Yanya He, Qingyuan Sun, Zhiwei Xue, Ziyi Tang, Junzhi Liu, Jiwei Wang, Chao Li, Xinyu Wang, et al.incubated with the primary antibody (anti-NUPR1 PA1-4177, Thermo Fisher Scientific, 1:100; anti-Ki67, 9027, Cell Signaling Technology, 1:200; anti-Lactyl Lysine, PTM-1401RM, PTM BIO, 1:400; anti-H4K8la, PTM-1415,

(13) Therapeutic synergies that overcome carboplatin resistance in triple-negative breast cancerJournal of Experimental & Clinical Cancer ResearchFebruary 3, 2026Julia E. Altman, Aaron Valentine, Nina Dashti-Gibson, Emily K. Zboril, David C. Boyd, Rachel K. Myrick, Amy L. Olex, Mikhail G. Dozmorov, J. Chuck HarrellM",40–68,1:1000,ThermoFisher Scientific,#PA1-27114 Ki-67,Rabbit IgG,H,,1:400,Cell Signaling Technology,#9027 Phospho-Histone H3,Rabbit IgG,"H, M, R, Mk, Dm",17,1:200,Cell Signaling Technology,#9701 UCHL1,IgG,"H,

(14) Identification of factors conferring resistance to trastuzumab deruxtecan in advanced gastric cancer: a translational study from the single-arm, phase II, DESTINY-Gastric06 trialPrecision Clinical MedicineJanuary 30, 2026Bohan Zhang, Lei Zhang, Cheng Liu, Tong Xie, Yifan Zhang, Xiao Wu, Yining Chen, Siyuan Cheng, Yang Feng, Yuxin Wang, et al.(Abcam, ab109508, 1:2000), CTSB (CST, CST31718, 1:1000), DAPI (Abcam, ab213363, 1:1000), and MKI67 (CST, 9027, 1:200). Negative control slides were processed with primary and secondary antibodies but without fluorophores

(15) CYLD Limits Neutrophil-Driven Psoriatic InflammationInflammationJanuary 20, 2026Zhenzong Fa, Zeping Huang, Yi Shang, Yang Yang, Qun Xie, Runping Yangindependent investigators. Primary antibodies used in this study: Ki67 (Cell signaling technology, 9027), Ly6G (Cell signaling technology, S7048T), CD3 (Abcam, ab16669), F4/80(Abcam, ab111101). RNA-seq analysis For

(16) ZNF384-regulated SLC31A1 expression promotes tumor proliferation and invasion in breast cancerMolecular and Cellular BiochemistryJanuary 19, 2026Tianzi Hong, Wenlian Li, Qingwei Wang, Zeyi Ye, Yueqin Xu, Bo Yang, Jiaxing Chen, Weibin Lian, Chuan Wangas a marker of cell proliferation. The slides were incubated with primary antibodies against Ki67 (9027, 1/200, Cell Signaling Technology) overnight at 4 °C, followed by incubation with HRP-conjugated secondary

(17) Targeting PLK1 Reduces MMP10 to Enhance Radiosensitivity in HPV— Head and Neck CancerClinical Cancer ResearchJanuary 16, 2026Julianna Korns, Maria A. Lehn, Shreya Shyamsunder, Mario Medvedovic, Stephanie M. Bryant, Mathieu G. Sertorio, Maya Ridinger, Trisha M. Wise-Draper, Vinita Takiarblocked (10% normal goat serum in 1X PBST) for 30 minutes. Primary antibody (Ki67, 1:500, CST Cat# 9027 RRID:AB_2636984) was diluted in green antibody diluent (Thermo Fisher, Cat# 3118) and incubated on tissues

(18) Molecular decoupling of lineage identity and morphology in aggressive variant prostate cancermedRxivJanuary 10, 2026Chennan Li, JuanJuan Yin, Melissa L. Abel, Dana S. Vargas Vargas Solivan, Kinjal Bhadresha, Sumeyra Kartal, Samantha Nichols, Kanak Parmar, Joseph Twohig, Tri M. Truong, et al.(Cell Signaling, cat. #3579), anti-ASCL1 (Cell Signaling, cat. #10585), anti-Ki-67 (Cell Signaling, cat. #9027), anti-E-cadherin (Cell Signaling, cat. #3195) and anti-cytokeratin 8 (Novus Biologicals, cat. #NBP2–16094),

(19) Targeting mTORC1 to promote ferroptosis and apoptosis in endometrial cancer with PI3K-Akt-mTOR pathway mutationFerroptosis and Oxidative StressDecember 29, 2025Yingying Hu, Pei Liu, Neal Rosen, Xuejun Jiangmicroscope. The following primary antibodies were used: Ki-67 (D2H10) Rabbit mAb (Cell Signaling Technology 9027S), 1:400; 4-Hydroxynonenal Antibody (R&D Systems MAB3249), 1:500; Cox2 (D5H5) XP® Rabbit mAb (Cell Signaling Technology

(20) Targeting CXCR6 Disrupts β-Catenin Signaling and Enhances Sorafenib Response in Hepatocellular CarcinomaCancers (Basel)November 28, 2025Morgan Reeves, Anastasia Chambers, Abhishek Shrestha, Sergio Duarte, Ali Zarrinpar, Siobhan Malany, Satyamaheshwar Peddibhotlaquenched with hydrogen peroxide, and sections were incubated with anti-Ki67 antibody (Cell Signaling, #9027; 1:250). Detection was performed with ImmPRESS horse anti-rabbit IgG, followed by chromogen development

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8/12/2023Reliable Ki67 Antibody From CST Dan ZhaoI used this antibody to stain proliferating cells for my MDAMB231 xenografted tumors, and it showed a very good signal on my tissue sections.

Ki-67 (D2H10) Rabbit mAb (IHC Specific)

Ki-67 (D2H10) Rabbit mAb from Cell Signaling Technology

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Ki-67 (D2H10) Rabbit mAb from Cell Signaling Technology

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