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APC anti-human CD38 Antibody
303510
HIT2
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B-cells in health and disease. Here, we aimed at staining plasma cells (CD27+CD38+) isolate from human bone marrow.
Flow cytometry
Human bone marrow from a total hip arthroplasty
Surface staining with CD38 APC, 20 min at 4 degrees C.
Fc block reagent
N/A
Flow cytometer
On the figure, on the left there is the expression of CD38 vs CD27 gated on CD14-CD3-dapi- (live B lineage cells). A very nice population of CD38hiCD27hi PCs can be observed. On the right there is an histogram of CD38 expression.
Reliable and good signal.
Great signal, highly recommended!