Abcam
Anti-HSF1 antibody [10H8]
ab61382
N/A
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Heat shock factor 1 (HSF1) is the main transcription factor mediating cellular responses under cytosolic stress conditions. Activation of HSF1 by post-translational modifications indicates a disturbance in protein homeostasis inside cells.
Western blot
Neuro2a cells
1:1000 in 1% BSA, Overnight at 4°C
5% BSA in Tris-buffered Saline Tween-20
1:5000 HRP-conjugated anti-Rat IgG, 1 hour at RT
ECL detection in documentation system (Vilber Lourmat)
HSF1 was activated at 8 hour of proteasome inhibition by MG132 treatment as observed in the gel by an upshifted phosphorylated protein band suggesting the loss protein homeostasis due to the proteotoxic stress.
https://doi.org/10.1016/j.jmb.2019.01.022
Antibody detects the band shift of phosphorylated HSF1.
Faint signal, and gives background spots.
Only purchase this antibody if there are no better options available that fit the experimental needs.