Abcam
Recombinant Anti-MTCO1 antibody [1D6E1A8]
ab14705
N/A
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MT-CO1 is one of the subunits of mitochondrial respiratory complex IV. We used it as a representative subunit to see distribution across various complex assemblies on a native-PAGE gel.
Western blot
Mitochondrial fraction from Neuro2a cells
1:1000 in 1% BSA, Overnight at 4°C
5% BSA in Tris-buffered Saline Tween-20
1:10000 HRP-conjugated anti-Mouse IgG, 1 hour at RT
ECL detection in documentation system. (Vilber Lourmat)
The antibody shows multiple bands corresponding to complex IV on a native gel. The individual complex IV (HC in the image) is the most prominent band whereas the higher bands (SC) indicate supercomplexes formed by interaction of complex IV with Complex III in different stoichiometries.
doi:10.1242/jcs.248492
Do not boil the samples (for native or sds page) to detect with this antibody. Read antibody manual carefully.
Good signal, it's a good antibody for a mitochondrial protein.
Requires careful optimization.
A good antibody for scientists working with mitochondrial biology.