Sigma-Aldrich
Z-Leu-Leu-Leu-al (MG132)
C2211-5MG
Info: Supplier Page
The ubiquitin-proteasome system is the main protein degradation machinery in cells. To study the effect of proteasome inhibition on the cellular proteome, I used Z-Leu-Leu-Leu-al, a synthetic peptide commonly known as MG132, that binds and blocks the 26S proteasome.
Cell culture treatment
Neuro2a cells
Aliquot and freeze for longer storage.
MG132 effectively blocks the ubiquitin-proteasome machinery as seen in the western blot with increase in ubiquitinated proteins.
https://doi.org/10.1016/j.jmb.2019.01.022
Its stability, and it is a cheaper alternative.
None.
Highly recommended, it is a stable and efficient proteasome inhibitor.