Mouse Anti-Human CD45RA From BD Biosciences Works Well

August 10, 2023

BlueskyReddit
Microbiology and Immunology
Columbia University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD OptiBuild™ BUV395 Mouse Anti-Human CD45RA

Catalog Number:

740298

Clone Number:

N/A

Info: Supplier Page 

Browse Similar Products: Anti-CD45RA Antibody Products

I used it for phenotyping human PBMC by flow cytometry. 5 lase Cytek Aurora was used and it works great.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human PBMC

Primary Incubation

1:200

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

I tittered the antibody and ended up using 1:200 dilution. Works great and separate positive population well.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

Expensive.

The Bottom Line

Expensive but great.

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