Sigma-Aldrich
Carbonyl cyanide 3-chlorophenylhydrazone (CCCP)
C2759
Info: Supplier Page
CCCP is a potent uncoupler of oxidative phosphorylation and disrupts the mitochondrial membrane potential. We used CCCP as a positive control for the mitochondrial protein import block.
Cell culture and microscopy
Neuro2a cells
Titrate concentration and incubation time for treatment as the effect of CCCP is reversible.
We transfected a GFP-based reporter that localizes to mitochondria and observed its localization using microscopy. CCCP affected the import of protein into mitochondria and GFP fluorescence was diffused in the cells, thus working as a positive control for mitochondria import block.
https://doi.org/10.1016/j.jmb.2019.01.022
Stability in solution.
Smaller pack size not available.
Good quality reagent with great stability and reproducibility.