Antibody From BD Biosciences With Very Strong IgM Staining

August 04, 2023

BlueskyReddit
Wayne State University

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BD Pharmingen™ FITC Mouse Anti-Human IgM

Catalog Number:

555782

Clone Number:

G20-127

Info: Supplier Page 

Browse Similar Products: Mouse Anti-Human Secondary Antibodies

Staining for IgM in human tonsils is very strong. Great antibody for studying B cells and plasma cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human tonsil

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Human tonsil cells stained with this antibody show very strong IgM signal. Positive staining appears as a smear, but the population is clearly separated.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining for B cells.

The Bad

N/A.

The Bottom Line

Staining of IgM on human B cells from tonsils is very strong. If you are analyzing human B cells, this antibody works well.

Share your experience with other scientists. Write a Review! »

Join the discussion