Great Antibody for VCAM-1 Staining From BioLegend

July 22, 2023

Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-mouse CD106 Antibody

Catalog Number:

105719

Clone Number:

429 (MVCAM.A)

Expression of VCAM-1 on mouse brain endothelial cells during viral respiratory infection was measured by flow cytometry.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse brain endothelial cells

Primary Incubation

1:100, 30 minutes on ice

Blocking Agent

TruStain FcX™ (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytoflex S flow cytometer (Beckman Coulter)

Results Summary

VCAM-1 expression on mouse brain endothelial cells is greatly increased during viral respiratory infection - indicating that brain endothelial cells are inflamed during infection of peripheral tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright signal.

The Bad

None.

The Bottom Line

This antibody stained primary mouse brain endothelial cells very well and was useful in identifying inflamed cells.

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