Great T-Cell Antibody for Flow Cytometry From BioLegend

July 22, 2023

BlueskyReddit
Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-mouse CD8a Antibody

Catalog Number:

100741

Clone Number:

53-6.7

CD8+ T-cell infiltration into the brain during viral respiratory infection in mice is assessed by flow cytometry.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse primary splenocytes and brain homogenate

Primary Incubation

1:50, 30 minutes on ice

Blocking Agent

TruStain FcX™ (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytoflex S flow cytometer (Beckman Coulter)

Results Summary

CD8+ T-cells were clearly stained in the spleen and a small population was identified in the brain of mice with viral respiratory infection.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal.

The Bad

High concentration of antibody needed for staining.

The Bottom Line

This antibody worked well to identify cytotoxic T-cells in multiple tissues by flow cytometry. The signal was bright and clear when a high concentration of antibody was used for staining.

Share your experience with other scientists. Write a Review! »

Join the discussion