Easy-to-Optimize Antibody for Surface Staining of HSP70

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

HSP70 Monoclonal Antibody (1H11), APC

Catalog Number:

MA5-45367

Clone Number:

1H11

This is an easy-to-optimize antibody for surface staining of Hsp70. The clone is unique for Hsp70 on tumor cells and therefore is useful for cancer studies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor cell lines

Primary Incubation

30 minutes, 4°C, in darkness

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This antibody helped to identify a discrete population of cells that express HSP70. This population was easily visible by flow cytometry and the signal was extremely clear. There was minimal optimization needed for surface staining and overall is a straightforward flow antibody to use for your studies. I'd highly recommend!

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Optimal clone, easy to titrate and stain.

The Bad

Would be nice to have more options for fluorophore-conjugation.

The Bottom Line

Antibody has great signal and ThermoFisher is the most affordable option for this particular clone.

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