Solid GFP Antibody From Thermo Fisher Scientific

July 12, 2023

BlueskyReddit
Biophysics and Biochemistry
University of California, San Francisco
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

GFP antibody

Catalog Number:

A11122

Clone Number:

N/A

Used for validating expression of APEX-GFP generated stable cell lines for the use of APEX- mass spectrometry.

Experimental Design and Results Summary

Applications

Western blot

Sample

HEK293

Primary Incubation

1:1000, 5% BSA, 4C o/n

Blocking Agent

3% milk

Secondary Incubation

3% milk, 1:1000

Tertiary Incubation

N/A

Detection

HRP

Results Summary

I was able to validate the expression of my plasma membrane and Golgi apparatus targeted APEX constructs for the generation of stable cells lines in HEK293.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy detection.

The Bad

No complaints.

The Bottom Line

Solid GFP antibody, worth buying.

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