FAS Antibody Works for Western Blot

June 22, 2023

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

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Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

Fatty Acid Synthase Antibody

Catalog Number:

sc-48357

Clone Number:

G-11

To test whether 25-OH cholesterol treatment in human THP-1 cell affects fatty acid synthase (FAS) expression. The results showed that 25-OH cholesterol did not change the FAS protein level.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human THP-1 cell samples

Primary Incubation

1:1000 overnight at 4 degree

Blocking Agent

5% milk in TBS

Secondary Incubation

1:3000, 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

To test whether 25-OH cholesterol treatment in human THP-1 cell affects fatty acid synthase (FAS) expression. The results showed that 25-OH cholesterol did not change the FAS protein level.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

FAS antibody works in human cell samples.

The Bad

N/A

The Bottom Line

Good antibody for Western Blot.

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