Cyto-Fast™ Fix/Perm Buffer Set from BioLegend

Product Specs
ItemCyto-Fast™ Fix/Perm Buffer Set
CompanyBioLegend
Price $276.00Supplier Page View Company Product Page
Catalog Number426803
Quantity300 tests
ApplicationsICFC - Quality tested

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) TRAIL splice variant TRAILshort disrupts T cell receptor signaling and promotes immune tolerance in vivoJ Clin InvestAugust 3, 2026Shahrzad Jalali, Sekar Natesampillai, Zilin Nie, Ying Zhang, Ismail Can, Aswath P. Chandrasekar, Brianna M. Hameister, Cristina Correia, Tuantuan V. Zhao, Dong-Gi Mun, et al.final 4 hours. Intracellular IFN-γ was detected using a commercial staining kit (BioLegend, catalog 426803) and analyzed by flow cytometry. IL-2 secretion. To evaluate IL-2 secretion in response to TRAILshort

(2) Engineering Immunoregenerative Therapy via an Immunomodulatory Binary Pharmacology Hydrogel Depot for Prolonged Allograft SurvivalAdv Sci (Weinh)July 7, 2026Ning Wang, Ruiqi Sun, Yang Fu, Zhonghan Wu, Xinyu Tong, Hong Tang, Wentao Zhao, Zhi Liang, Jintao Zheng, Yanan Guan, et al.cocktail (423304, BioLegend) for 4 h, fixed and permeabilized using the Cyto‐fast fix/perm buffer kit (426803, BioLegend), and stained intracellularly with PE‐Cy7 anti‐mouse granzyme B (372214, BioLegend) and APC anti‐mouse perforin

(3) FATP2-mediated lipid metabolism enhances chimeric antigen receptor T-cell therapy resistance in B-cell acute lymphoblastic leukemiaLeukemiaJune 30, 2026Clarissa Garcia, Kaylyn Utley Lyons, Julian Grandvallet Contreras, Tian Liu, Alexis J. Donnelly, Amanda J. Novak, Amy Argabright, Colin Anderson, Abby Grier, Sabrina Smith, et al.assays were performed using DAPI counterstaining following BioLegend Cyto-Fast Fix/Perm Buffer set (Cat# 426803). For BODIPY uptake assays, we serum-starved B-ALL in RPMI 1640 media for 1 h; then added 1 μM long-chain

(4) NRF2-mediated inhibition of alveolar macrophage MHC II expression during Mycobacterium tuberculosis infectionCell ReportsJune 23, 2026Linh K. Pham, Maritza M. Cervantes, Pamelia N. Lim, Divya Dubey, Ankita Tufts, M. Clare Mitchell, Tomoyo Shinkawa, Samuel M. Behar, Alissa C. Rothchildproteins" ESAT63-17,Genscript,Sequence: TEQQWNFAGIEAAASAI Cyto-Fast™ Fix/Perm Buffer Set,BioLegend,Cat# 426803 Paraformaldehyde Aqueous Solution (20%) EM Grade,Electron Microscopy Sciences,Cat# 15713-S Phosphate

(5) Comprehensive mapping of human CD4+ T cell epitopes for Nipah and measles as prototype ParamyxovirusesCell Rep MedJune 16, 2026Alison Tarke, Mariah Macias, Claudia Francisco Morales, Tanner Michaelis, Leila Siddiqui, Esther Dawen Yu, Raphael Trevizani, Abril Zuniga, Christian Zmasek, Elizabeth Phillips, et al.Aqua Stain Kit,Thermo Fisher Scientific,Cat#: L34966 Cyto-Fast Fix/Perm Buffer Set,Biolegend,Cat#: 426803 FluoroSpot Enhancer,Mabtech,Cat#: 3641-F1 Synthetic peptides,TC Peptide Lab,N/A ,, Critical commercial

(6) Decoding the human CD4+ T cell epitope repertoire for Lassa fever virus reveals T cell-based pan-mammarenavirus vaccine candidatesCell Reports MedicineJune 16, 2026Yeji Lee, Leila Siddiqui, Alison Tarke, Margaret Kim, Tanner Michaelis, Mariah Macias, Elizabeth Phillips, Simon Mallal, Raphael Trevizani, April Frazier, et al.Buffer Plus,BD Biosciences,Cat# 566385;RRID: AB_2869761 Cyto-Fast Fix/Perm Buffer Set,BioLegend,Cat# 426803 AddaVax adjuvant,InvivoGen,Cat# vac-adx-10 ,, Critical commercial assays,Critical commercial assays,Critical

(7) Efficacy and immunogenic effects of Tumor Treating Fields ( TTFields ) in preclinical models of pancreatic ductal adenocarcinoma, with and without gemcitabine/nab‐paclitaxelInternational Journal of CancerJune 15, 2026Tal Kan, Tharwat Haj Khalil, Yiftah Barsheshet, Tali Voloshin, Lilach Koren, Bella Koltun, Cfir David, Kerem Wainer‐Katsir, Avital Vorontsova, Boris Brant, et al.(108724, BioLegend), and MHC class II (MHC‐II)‐BV510 (107635, BioLegend). Cyto‐Fast™ Fix/Perm Buffer Set (426803, BioLegend) was utilized according to manufacturer instructions. Data was acquired on Cytek® Northern

(8) Deleterious germline CARD11 gain-of-function variants alter human B-cell and CD4+ T-cell differentiation and functionClin Exp ImmunolJune 12, 2026Tina Nguyen, Melissa A Kallarakal, Jackie L Ludgate, Ian M Morison, Helen C Su, Swadinhya Arjunaraja, Andrew L Snow, Cindy S Ma, Stuart G TangyeBV750; clone: FN50) mAbs. Cells were fixed/permed with the CytoFast Fix/Perm Buffer Kit (Biolegend: 426803) and stained intracellularly with anti-pS6 mAb (clone: D57.2.2E). Flow cytometry data were collected

(9) LPAR4 mediates resistance to interferon-induced stress in soft tissue sarcomaSci AdvJune 12, 2026Jin-Fen Xiao, Emily Y. Ko, Ashley Smith, Roberta Piras, Annaliese Fowler, Kristin Ishaya, Longqiang Wang, Bin Wang, Adriana Paulucci-Holthauzen, Sharon M. Landers, et al.Briefly, cells were harvested and then fixed and permeabilized using the Cyto-Fast Fix/Perm Buffer Set (426803, BioLegend) according to the manufacturer’s instructions. dsRNA was probed with 2 μg of SCICONS J2 anti-dsRNA

(10) Classification of recurrent implantation failure patients using peripheral blood immunological and metabolic markersBioimpactsJune 9, 2026Sina Baharaghdam, Özkan Yildirim, Mohammadbagher Pirouzpanah, Danial Abbas Kabiri, Shima Karimi, Mohammad Esfini Farahani, Amirhossein Aghapour, Ali Hassanzadeh, Amin Kamrani, Reza Mousavi Ardehaie, et al.permeabilization using the Fixation/Permeabilization Solution Kit (Biolegend, San Diego, CA, USA; Cat. No. 426803), in accordance with the manufacturer’s protocol. On a BD FACSCalibur (BD Biosciences), at least 50,000

(11) Systemic pre-conditioning favors effector over exhausted CD8 T-cell subsets following Sup2-IL33 armored CAR T-cell therapyJ Immunother CancerJune 3, 2026Shannon L Ferry, Leena Abdullah, Akshaya Balasubramanian, Elizabeth C Nowak, Allison Carl, Alexandrea Turnquist, Asmaa Mohamed, Edward Usherwood, Mark Sundrud, Yina Huangpreviously described, then fixed, permeabilized, and stained with antibodies for cytokines (BioLegend 426803). Multiplex Immunohistochemistry (IHC) Tumor were fixed in 10% neutral balanced formalin, embedded

(12) Identification of the critical immune-related drivers shared by non-alcoholic fatty liver disease and osteoporosisCellular and Molecular Life SciencesMay 23, 2026Feifan Chang, Siliang Ge, Mingming Zhang, Fanfeng Wu, Fuming Cao, Jiang Liu, Mingxing Lei, Zhikang Guo, Wenbo Tang, Ming Chen, et al.and permeabilization were carried out at room temperature using the Cyto-Fast™ Fix/Perm Buffer Set (426803, Biolegend, USA). PE anti-mouse IL-4 (504104, Biolegend, USA), APC anti-mouse IFN-γ (505810, Biolegend,

(13) Membrane-bound TRAIL-armoring augments CAR-T cells in mesothelin-positive solid malignanciesJ Immunother CancerMay 21, 2026Alessia Volpe, Juan Zurita, Larissa Shenker, Mayuresh M Mane, Prasad S Adusumilli, Alexander Aleshin, Dmitri Rozanov, Vladimir PonomarevCAR+cells was assessed by intracellular staining using a Cyto-Fast Fix/Perm buffer set (BioLegend #426803). Experimental design was identical to monolayer killing assay. In addition, CAR-T cells were treated

(14) Disruption of Treg Homeostasis in Rheumatoid Arthritis via Ferroptosis‐Mediated ETC Collapse and TXK‐STAT3/PLCγ1 ActivationAdvanced ScienceMay 19, 2026Jingrong Chen, Xiao Guan, Kexu Xiong, Xiaoyi Shi, Luyao Wu, Chuanxin Su, Jun Zhao, Rongzhen Liang, Huimin Wang, Junlong Dang, et al.fixed and permeabilized according to instructions for the Cyto‐Fast Fix/Perm Buffer Set (BioLegend 426803). Flow cytometry data collected on a Cytek Northern Lights platform were processed in FlowJo. 4.16

(15) Feeder-free generation of functional dendritic cells from human pluripotent stem cellsJ Immunother CancerMay 13, 2026Wei-Hung Jung, Andrew Khalil, Yoav Binenbaum, Joshua M Price, Kyle H Vining, Tenzin Lungjangwa, Miguel C Sobral, Rudolf Jaenisch, David J Mooneyextracellular staining, intracellular cytokine staining was performed using the Cyto-Fast Fix/Perm Buffer Set (BioLegend, 426803) according to the manufacturer’s instructions, followed by flow cytometric analysis. Design of experiments A

(16) Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complexPLoS PathogApril 20, 2026Stefano Sellitto, Davide Caredio, Matteo Bimbati, Giovanni Mariutti, Martina Cerisoli, Lukas Frick, Vangelis Bouris, Carlos Omar Oueslati Morales, Dalila Laura Vena, Sandesh Neupane, et al.For flow cytometry, HovL cells were dissociated and fixed using the Cyto-Fast Fix/Perm buffer set (BioLegend, 426803) according to the manufacturer’s instructions. Additionally, after fixation, samples were incubated

(17) γδ T cell-stromal networks modulate matrix composition and vascularity in foreign body responseNature CommunicationsApril 17, 2026Anna Ruta, Kavita Krishnan, JiWon Woo, Joscelyn C. Mejías, Elise F. Gray-Gaillard, David R. Maestas Jr., Helen Hieu Nguyen, Alexandra N. Rindone, Christopher Cherry, Michael Patatanian, et al.detailed above. Stained cells were fixed and permeabilized using Cyto-fast fix/perm solution (BioLegend 426803) for 20 min covered at RT, followed by washing with 1x Cyto-fast perm wash solution, and ICS for 20

(18) Genome-wide CRISPR screens in primary human natural killer cells identify countermeasures against immunosuppressive environmentNature CommunicationsApril 15, 2026Quoc Viet Nguyen, Yi-Jun Lan, Jason Cheng-Yu Chang, Hsin-An Shih, Jenifer Faustine, Cheng-Chieh Chen, Shu-Yu Ho, Ching-Wen Cheng, Tsu-Lan Chao, Steven Linstaining, cells were fixed and permeabilized using 100 μL of Cyto-Fast Fix/Perm Buffer Set (BioLegend # 426803) at room temperature for 20 minutes, washed twice with Cyto-Fast Perm Wash Solution, and stained with

(19) Histotripsy-Initiated Immune Response Synergizes with Chemotherapy in a Neuroblastoma Murine ModelCancers (Basel)April 15, 2026Natalia Antonides-Jensen, Muskan Singh, Yuqing Xue, Fernando Flores-Guzman, Lydia L. Wu, Samantha S. Yee, Jacky Gomez-Villa, Timothy L. Hall, Mark A. Applebaum, Kenneth B. Bader, et al.fixed and permeabilized with CytoFast Fix/Perm Buffer Set (BioLegend, San Diego, CA, USA; cat. no. 426803) and stained with the panel of antibodies described in Supplementary Table S1. Analyses were performed

(20) Cancer systems immunology reveals myeloid—T cell interactions and B cell activation mediate response to checkpoint inhibition in metastatic breast cancerbioRxivApril 12, 2026Edgar Gonzalez, Jesse Kreger, Yingtong Liu, Xiaojun Wu, Arianna Barbetta, Aaron G. Baugh, Batul Al-Zubeidy, Julie Jang, Sarah M. Shin, Matthew Jacobo, et al.Buffer Set (eBioscience™, cat. #00-5523-00) or the Cyto-Fast™ Fix/Perm buffer set (Biolegend, cat. # 426803) following the manufacturer’s directions prior to a 10-minute FcR block step at room temperature and

Write a Review

(1)

3/1/2024Readily Available Fix and Perm Buffer for Flow Cytometry Applications Wei Yang SitThe kit is designed for fixation and permeabilization of mammalian cells for intracellular staining such as cytokines and other cytoplasmic molecules.
(1)

6/1/2023Buffer Set Helps Make Cells Permeable for Intracellular Staining Soheil TavakolpourThis product has both Cyto-Fast™ Fix Perm Solution and Cyto-Fast™ Perm Wash Solution (10X). It can be used for fixation and permeabilization of mammalian cells for intracellular staining, such as ... Read Review

Cyto-Fast™ Fix/Perm Buffer Set from BioLegend

Description

BioLegend

Citations (201)

Reviews

Cyto-Fast™ Fix/Perm Buffer Set from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map