Cyto-Fast™ Fix/Perm Buffer Set from BioLegend

Product Specs
ItemCyto-Fast™ Fix/Perm Buffer Set
CompanyBioLegend
Price $276.00Supplier Page View Company Product Page
Catalog Number426803
Quantity300T
ApplicationsICFC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

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Website: biolegend.com

(1) P2X7R+CD8αβ+ T cells exacerbate postoperative ileus by inducing the loss of enterochromaffin cellsiScienceOctober 16, 2026Fandi Bu, Shiyang Huang, Xiaobao Yang, Luyang Wei, Yingchi Yang, Zhongtao Zhang, Dong Zhang, Dan TianCell Activation Cocktail (with Brefeldin A),Biolegend,Cat#423304 Cyto-Fast Fix/Perm Buffer Set,Biolegend,Cat#426803 Anti-PE MicroBeads,Miltenyi Biotec,Cat#130–048–801 EasySep Magnet,STEMCELL Technologies,Cat#18000 RNeasy

(2) Vitamin B12 Alleviates Hepatic Ischemia–Reperfusion Injury with Restoration of Propanoate Metabolism and Mitochondrial FunctionJ Inflamm ResSeptember 24, 2026Simeng Lei, Zhenyu Zheng, Hao Wang, Hanwen Yang, Yuepeng Wang, Bo Zhang, Yangkai Fu, Zhen Li, Zhili Ji(MP5-20F3, BioLegend), IL-1β (12–7114-82, eBioscience), a rapid cell fixation/permeabilization buffer kit (426803, BioLegend), and a cell activation mixture (423304, BioLegend). Hepatocyte proliferation was assessed

(3) Enrichment of CD4-CD8- cytotoxic Vδ1/3 T cells in pulmonary tuberculosis diseaseiScienceSeptember 18, 2026Kendall Kearns, Rashmi Tippalagama, Ashu Chawla, Jason Greenbaum, Aruna Dharshan De Silva, Wathsala Gunasinghe, Judy Perera, Hansani Gunasekera, Darsha D. Senevirathne, Thomas J. Scriba, et al.assays,Critical commercial assays,Critical commercial assays ,, Cyto-Fast Fix/Perm Buffer Set,BioLegend,Cat#: 426803 ,, Deposited data,Deposited data,Deposited data ,, 10× sequencing data,This paper,GEO: GSE312606 Flow cytometry

(4) Lactobacillus salivarius potentiates gastrointestinal cancer immunotherapy through its metabolite chenodeoxycholic acidCell Reports MedicineSeptember 15, 2026Bohan Zhang, Yifan Zhang, Yi Cai, Siyuan Cheng, Zihan Han, Xiaochen Yin, Tong Xie, Yan Tan, Yang Feng, Yuxin Wang, et al.and Sharpe (MRS) medium",This paper,N/A LB medium,This paper,N/A Permeabilization kit,BioLegend,Cat#426803 Anti-mouse CD3/CD28 Dynabeads,STEMCELL,Cat#100-1572 PMA and Ionomycin,Dakewe,Cat#2030421 Dextran

(5) Targeting of tripartite neuron-cancer-immune cell crosstalk augments response to chemotherapy and immunotherapyCell Reports MedicineSeptember 15, 2026Dolly Jain, Nishant Pandey, Somesh K. Jha, Shreya Sinha, Ragini Singh, Junaid Alam, Bharti Aggarwal, Neelam Chauhan, Arunima Acharya, Anu P.V., et al.423102 Recombinant Mouse IFN-γ,BioLegend,Cat# 575306 Cyto-Fast™ Fix/Perm Buffer Set,BioLegend,Cat# 426803 Recombinant Human IFN-γ,BioLegend,Cat# 570206 Recombinant Mouse IL-2,BioLegend,Cat# 575404 Recombinant

(6) Depletion of an immature cord blood NK subset reverses trogocytosis-driven CAR NK dysfunctionCancer CellSeptember 14, 2026Ye Li, Huihui Fan, Guohui Wang, Xin Wang, Rafet Basar, Maliha Nuzhat Munir, Nadima Uprety, Rejeena Shrestha, Ludovica La Posta, Bin Liu, et al.solution 500 mL,Agilent Technologies,Cat# 100840-000 Cyto-Fast Fix/Perm buffer set,BioLegend,Cat# 426803 FuGENE HD transfection reagent,Fugene HD,Cat# E2312 Applied biosystems fast SYBR green master mix,Applied Biosystems

(7) Early-onset CLL is characterized by enhanced microenvironment-driven metabolic fitness, increased proliferative bias, and accelerated disease progressionFront ImmunolSeptember 7, 2026Eugenia Payque, Santiago A. Rodríguez-Zraquia, Marianela Lasagna, Rita Uria, Vanesa A. Guazzone, Victoria Irigoin, Ana Inés Landoni, Victoria Remedi, Virginia Lema, Sabrina Ranero, et al.antibodies,Flow cytometry antibodies,Flow cytometry antibodies Cyto-Fast™ Fix/Perm Buffer Set,Biolegend,Cat#426803 CD5-PE-Cy7,Biolegend,Cat: 300622 CD19-PE,Biolegend,Cat: 115508 CD19- Pacific Blue,Biolegend,Cat:

(8) Unc-51 like kinase 3 (ULK3) contributes to autophagy and cell survival in multiple myelomaNature CommunicationsAugust 25, 2026Marilena Tauro, Tao Li, Praneeth R. Sudalagunta, Mark Meads, Rafael Renatino Canevarolo, Niveditha Nerlakanti, Raghunandan R. Alugubelli, Harshani R. Lawrence, Mukund P. Tantak, Steven Gunawan, et al.followed by fixation and permeabilization steps with Cyto-Fast™ Fix/Perm Buffer Set (Biolegend; Cat# 426803) according to the manufacturer’s instructions. Cells were then stained with Alexa Fluor® 647 Anti ULK3 antibody [EPR4888

(9) Targeting the NR1D1-IGF2BP2-V-ATPase Axis With Hybrid Nanovesicles Restores Macrophage Rhythms to Reverse Sepsis-Induced ImmunosuppressionAdv Sci (Weinh)August 24, 2026Lang Chen, Wenyi Lin, Lang Jiang, Xuehui Gao, Chenyan Yu, Jili Han, Zhenping Liu, Xuefeng Li, Mei He, Chang Li, et al.detection, cells were fixed and permeabilized using the Fixation/Permeabilization Solution Kit (no. 426803, BioLegend) before staining with Alexa Fluor 647‐CD206 (no. 141712, BioLegend). Samples were acquired

(10) Immune Circuit Rewiring by HDAC Inhibition Enables Response to Dual Checkpoint Blockade in Metastatic Breast CancerCancer ResAugust 11, 2026Edgar Gonzalez, Jesse Kreger, Yingtong Liu, Xiaojun Wu, Arianna Barbetta, Aaron G. Baugh, Batul Al-Zubeidy, Julie K. Jang, Sarah M. Shin, Zhehao Zhang, et al.Buffer Set (eBioscience−, cat. #00-5523-00) or the Cyto-Fast− Fix/Perm buffer set (Biolegend, cat. #426803) following the manufacturer’s directions prior to a 10-minute FcR block step at room temperature and

(11) Regulatory B cells contribute to allergen-encapsulating nanoparticle immunotherapy efficacy for food allergyJCI InsightAugust 10, 2026Laila M. Rad, Michael N. Saunders, Laura A. Williams, Katarzyna W. Janczak, Chris L. Dorsett, Kate V. Griffin, Elizabeth J. Bealer, Jeffrey A. Ma, Sayre A. Tillery, Jyotirmoy Roy, et al.culture medium. Intracellular cytokine staining was done using Cyto-Fast Fix/Perm buffer set (BioLegend, 426803) following the manufacturer’s instructions. Bulk RNA-Seq of splenic and Peyer’s patch B cells. Mice

(12) Macrophages tune NK cell IFNγ production through direct lipid transferNature CommunicationsAugust 8, 2026Cathal Keane, Shauna Mayock, Carrie Corkish, Ella Rogerson, Cristhiane Favero de Aguiar, Ya Gao, Sarah Kenny, Lorraine Brennan, Suzanne M. Cloonan, David K. Finlaystains were done at 4 °C for 20 min in the dark. Cells were fixed with Cyto-FAt Fix-Perm Buffer Set (426803, BioLegend) and stained intracellular in corresponding buffer. Where cytokine staining was required,

(13) Follicular Lymphoma Transformation is Characterized by Cytokine-associated Remodeling of Stromal and Macrophage CompartmentsCancer DiscovAugust 4, 2026Nicholas J Haradhvala, Stephanie Pei Tung Yiu, Laura Beckmann, Sam Sadigh, Zachary Leibowitz, Erik Landolsi, Santiago Rivero, Stephanie L. Deng, Sabrina Hu, Mingzeng Zhang, et al.for intracellular staining were performed using the Cyto Fast Fix/Perm Buffer Set (Biolegend, cat. 426803) following the manufacturer’s protocol. For intracellular staining, PE-conjugated anti-SPP1 antibody

(14) TRAIL splice variant TRAILshort disrupts T cell receptor signaling and promotes immune tolerance in vivoJ Clin InvestAugust 3, 2026Shahrzad Jalali, Sekar Natesampillai, Zilin Nie, Ying Zhang, Ismail Can, Aswath P. Chandrasekar, Brianna M. Hameister, Cristina Correia, Tuantuan V. Zhao, Dong-Gi Mun, et al.final 4 hours. Intracellular IFN-γ was detected using a commercial staining kit (BioLegend, catalog 426803) and analyzed by flow cytometry. IL-2 secretion. To evaluate IL-2 secretion in response to TRAILshort

(15) RelB drives integrin-mediated stress tolerance and relapse in high-grade serous ovarian cancerCell ReportsJuly 28, 2026Omar Lujano-Olazaba, Mikella Robinson, Gregory J. Jordan, Sofia Howe, Anna Platen, Cassidy Lucht, Dishant Vandra, Cecilia Gallo, Mena Shammas, Katelyn Shelby, et al."CD117 MicroBead Kit, human (AC126)",Miltenyi Biotech,Cat#130-091-332 Cyto-Fast Fix/Perm Buffer Set,BioLegend,Cat#426803 FcR Blocking Reagent,Miltenyi Biotech,Cat#130-059-901 OneMinute® Advance WB Stripping Buffer,GM Biosciences,Cat#GM6031 Deposited

(16) TDP-43 promotes allergen-induced inflammation and influenza A virus replication in airway epitheliumNature CommunicationsJuly 23, 2026Yanmei Yuan, Wenzhu Tang, Xixi Feng, Chunlei Zhang, Yingying Liao, Meiyan Tang, Jian He, Shengpeng Wan, Guihong Pan, Xiaojiang Lai, et al.flow panels. For intracellular staining, the cells were treated with Cyto-Fast Fix/Perm Buffer Set (426803, BioLegend) and stained with anti-mouse IL-4 (504126, BioLegend), IL-5 (504306, BioLegend), IL-13 (159403,

(17) Engineering Immunoregenerative Therapy via an Immunomodulatory Binary Pharmacology Hydrogel Depot for Prolonged Allograft SurvivalAdv Sci (Weinh)July 7, 2026Ning Wang, Ruiqi Sun, Yang Fu, Zhonghan Wu, Xinyu Tong, Hong Tang, Wentao Zhao, Zhi Liang, Jintao Zheng, Yanan Guan, et al.cocktail (423304, BioLegend) for 4 h, fixed and permeabilized using the Cyto‐fast fix/perm buffer kit (426803, BioLegend), and stained intracellularly with PE‐Cy7 anti‐mouse granzyme B (372214, BioLegend) and APC anti‐mouse perforin

(18) FATP2-mediated lipid metabolism enhances chimeric antigen receptor T-cell therapy resistance in B-cell acute lymphoblastic leukemiaLeukemiaJune 30, 2026Clarissa Garcia, Kaylyn Utley Lyons, Julian Grandvallet Contreras, Tian Liu, Alexis J. Donnelly, Amanda J. Novak, Amy Argabright, Colin Anderson, Abby Grier, Sabrina Smith, et al.assays were performed using DAPI counterstaining following BioLegend Cyto-Fast Fix/Perm Buffer set (Cat# 426803). For BODIPY uptake assays, we serum-starved B-ALL in RPMI 1640 media for 1 h; then added 1 μM long-chain

(19) NRF2-mediated inhibition of alveolar macrophage MHC II expression during Mycobacterium tuberculosis infectionCell ReportsJune 23, 2026Linh K. Pham, Maritza M. Cervantes, Pamelia N. Lim, Divya Dubey, Ankita Tufts, M. Clare Mitchell, Tomoyo Shinkawa, Samuel M. Behar, Alissa C. Rothchildproteins" ESAT63-17,Genscript,Sequence: TEQQWNFAGIEAAASAI Cyto-Fast™ Fix/Perm Buffer Set,BioLegend,Cat# 426803 Paraformaldehyde Aqueous Solution (20%) EM Grade,Electron Microscopy Sciences,Cat# 15713-S Phosphate

(20) Comprehensive mapping of human CD4+ T cell epitopes for Nipah and measles as prototype ParamyxovirusesCell Rep MedJune 16, 2026Alison Tarke, Mariah Macias, Claudia Francisco Morales, Tanner Michaelis, Leila Siddiqui, Esther Dawen Yu, Raphael Trevizani, Abril Zuniga, Christian Zmasek, Elizabeth Phillips, et al.Aqua Stain Kit,Thermo Fisher Scientific,Cat#: L34966 Cyto-Fast Fix/Perm Buffer Set,Biolegend,Cat#: 426803 FluoroSpot Enhancer,Mabtech,Cat#: 3641-F1 Synthetic peptides,TC Peptide Lab,N/A ,, Critical commercial

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(1)

3/1/2024Readily Available Fix and Perm Buffer for Flow Cytometry Applications Wei Yang SitThe kit is designed for fixation and permeabilization of mammalian cells for intracellular staining such as cytokines and other cytoplasmic molecules.
(1)

6/1/2023Buffer Set Helps Make Cells Permeable for Intracellular Staining Soheil TavakolpourThis product has both Cyto-Fast™ Fix Perm Solution and Cyto-Fast™ Perm Wash Solution (10X). It can be used for fixation and permeabilization of mammalian cells for intracellular staining, such as ... Read Review

Cyto-Fast™ Fix/Perm Buffer Set from BioLegend

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Cyto-Fast™ Fix/Perm Buffer Set from BioLegend

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