Good Neuron-Specific Enolase for Some Species

Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Agilent

Product Name:

Neuron-Specific Enolase (NSE)

Catalog Number:

IR612

Clone Number:

BBS/NC/VI-H14

Info:Supplier Page View Product Specs

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This NSE antibody is working on cat tissues very well. But not so good on canine tissues. I got weak results on dog tissues.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Cat intestine

Primary Incubation

RTU

Blocking Agent

BSA, 1%

Secondary Incubation

Anti-mouse IgG, RTU

Tertiary Incubation

Peroxidase-strepteavidin complex (1 hours at RT) and tehn AEC chromogen, RT 20'

Detection

ABC-peroxidase technique

Results Summary

Antibody is giving great signalling. No background staining, No nonspecific staining. Strong specific signalling. I'm using it neuro-endocrine tumour suspected feline tumours. It is useful also for embryogenesis work. Image legend: strong positivity on Auerbach plexuses and intramuscular nerves (arrows) on feline intestine.

DOI or PMID #

N/A

Additional Notes

Gill's hemalum as a background staining.

Image Gallery

Summary

The Good

Easy to use and good for feline tissues. less background.

The Bad

Weak staining on canine tissues.

The Bottom Line

The antibody that I use was RTU. So with concentrate one maybe higher dilution can use for other species.

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