Good P-SMAD2 Antibody for Immunostaining

Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

P-SMAD2

Catalog Number:

AB3849-1

Clone Number:

N/A

Info:Supplier Page 

Browse Similar Products: SMAD2 Antibodies

Smad proteins SMAD2 and SMAD3 are core transcription factors involved in TGF-beta signaling, and they interact with cofactors to control the expression of TGF-beta-dependent genes. The principal receptor-activated Smads involved in signaling from TGF-β receptors are Smad2 and Smad3. In the nucleus, Smad proteins can bind directly to their cognate DNA-binding sites and/or interact with an increasing number of transcription factors, transcriptional coactivators, or transcriptional repressors.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue liver and Heart

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

1% BSA prepared in 1X TBST

Secondary Incubation

1:250 dilution 1% BSA prepared in 1X TBST for 2hrs

Tertiary Incubation

None

Detection

Fluorescent microscope

Results Summary

Smad2 and Smad3 are similarly proposed to act downstream of TGFβ signaling. in our results we found mouse tissue heart samples increased p-SMAD2 levels.

DOI or PMID #

NA

Additional Notes

N/A

Image Gallery

Summary

The Good

Great

The Bad

None

The Bottom Line

Highly recommended to this immunostaing.

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