Santa Cruz Biotechnology
Insulin antibody
SC-8033
2D11-H5
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I use this antibody in my diabetes study with streptozotocin. It stained undamaged Beta cells very strongly by immunohistochemistry. almost it makes zero background staining. Sharp and clear. Thus, exact numbers of data were obtained from the insulin stained ihc photographs.
Immunohistochemistry
Rat pancreas tissue
1/50 dilution, diluent BSA %1, 2 hours at RT
%1 BSA
biotinylated anti-mouse IgG
peroxidase conjugated streptavidin - AEC chromogen
ABC-method
In my diabetes studies, it enabled the detection of healthy and insulin-producing Beta cells in the pancreas.
Gill's hemalum as a background staining.
specific binding and without background staining
no bad things...
The antibody has become the standard insulin of the my laboratory. I recommend it to anyone studying insulin immunohistochemistry.