New England Biolabs
Phusion® Hot Start Flex 2X Master Mix
M0536S
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This master mix enables researcher to minimize the time for optimization of PCR reactions, It helps to start PCR with high Tm and minimize the non-specific products.
Conventional PCR
N/A
It is better to add the enzyme after 5 minutes of DNA denaturation (for genomic DNA)
The best results could be achieved by optimization of the reaction. However, touchdown PCR can help to get the product easier.
No non-specific amplification.
Expensive
In general, it is worth using this master mix for any type of PCR reaction. It is one of the best option for doing the genotyping.