Phusion Hot Start Flex 2X Master Mix M0536S from New England Biolabs

Product Specs
ItemPhusion Hot Start Flex 2X Master Mix
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0536S
Quantity100 reactions
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Optimized single-cell RNA sequencing protocol to study early genome activation in mammalian preimplantation developmentSTAR ProtocolsSeptember 1, 2023Nina Boskovic, Gamze Yazgeldi, Sini Ezer, Mari H. Tervaniemi, Jose Inzunza, Spyridon Panagiotis Deligiannis, Barış Yaşar, Tiina Skoog, Kaarel Krjutškov, Shintaro Katayama, et al.10×",NEB,Cat# B6059S "Klenow exo, 5 U/μL",NEB,Cat# M0212S Phusion Hot Start Flex 2× Master Mix,NEB,Cat# M0536S "T4 DNA ligase, 5 U/μL",Thermo Scientific,Cat# EL0014 Terra PCR Direct Polymerase Mix,Takara,Cat#

(2) Repurposing CRISPR/Cas to Discover SARS‐CoV‐2 Detecting and Neutralizing AptamersAdvanced ScienceMay 19, 2023Ju Zhang, Airu Zhu, Miao Mei, Jing Qu, Yalan Huang, Yongshi Shi, Meiying Xue, Jingfang Zhang, Renli Zhang, Bing Zhou, et al.byproducts. The high‐fidelity polymerase Phusion Hot Start Flex 2 × Master Mix (NEB, Ipswich, USA, M0536S) was used for PCR. Then the PCR reaction was run on a 2% agarose gel, and product ≈140 bp was purified

(3) Achieving single nucleotide sensitivity in direct hybridization genome imagingNature CommunicationsDecember 15, 2022Yanbo Wang, W. Taylor Cottle, Haobo Wang, Momcilo Gavrilov, Roger S. Zou, Minh-Tam Pham, Srinivasan Yegnasubramanian, Scott Bailey, Taekjip Hasubstrates for in vitro cleavage assays were synthesized using Phusion® Hot Start Flex 2X Master Mix (NEB, M0536S) and purified using GeneJET PCR Purification Kit (Thermo Scientific, K0701). The primers were purchase

(4) The timing of differentiation and potency of CD8 effector function is set by RNA binding proteinsNature CommunicationsDecember 1, 2022Georg Petkau et al.PCR amplification was conducted using P5 and P3 solexa primers [Illumina] and Phusion HF master mix [NEB M0536S]. PCR products were run on 6% TBE gel and DNA in the range of 145-400 nt isolated using standard methodology.

(5) In vivo mitochondrial base editing via adeno-associated viral delivery to mouse post-mitotic tissueNature CommunicationsDecember 1, 2022Pedro Silva-Pinheiro, Pavel A. Nash, Lindsey Van Haute, Christian D. Mutti, Keira Turner, Michal Minczukindex and sequencing primers, in a 25 µl reaction, with Phusion® Hot Start Flex 2X Master Mix (NEB, M0536S) using the following primers: Nd3adapter_Fw: 5’– TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG TTC TGA

(6) Atypical cyclic di-AMP signaling is essential for Porphyromonas gingivalis growth and regulation of cell envelope homeostasis and virulencenpj Biofilms and MicrobiomesDecember 1, 2022M. Fata Moradali, Shirin Ghods, Heike Bähre, Richard J. Lamont, David A. Scott, Roland SeifertTable 2. The PCR was performed using Phusion Hot Start Flex 2X Master Mix (New England BioLabs; cat# M0536S) and consisted of 25 cycles with a temperature profile of 30 s at 98 °C, 30 seconds at 57 °C for annealing,

(7) Genome Oligopaint via Local Denaturation Fluorescence in Situ Hybridization (GOLD FISH)Molecular CellApril 1, 2021Yanbo Wang, Wayne Taylor Cottle, Haobo Wang, Xinyu Ashlee Feng, John Mallon, Momcilo Gavrilov, Scott Bailey, Taekjip Ha(Twist Bioscience) was amplified to a dsDNA pool using Phusion® Hot Start Flex 2X Master Mix (NEB, M0536S) by limit-cycle PCR (no more than 10 cycles). The crRNAs for ChrX ‘paint’ was in vitro transcribed using

(8) Binary Fate Choice between Closely Related Interneuronal Types Is Determined by a Fezf1-Dependent Postmitotic Transcriptional SwitchNeuronFebruary 1, 2020Yi-Rong Peng, Rebecca E. James, Wenjun Yan, Jeremy N. Kay, Alex L. Kolodkin, Joshua R. SanesNEBuilder® HiFi DNA Assembly Cloning Kit,New England BioLabs,Cat# E5520S Phusion® Hot Start Flex 2X Master Mix,New England BioLabs,Cat# M0536S SuperScript™ III First-Strand Synthesis SuperMix,ThermoFisher,Cat# 18080400 TRIzol™ Plus RNA Purification Kit

(9) Endosome-Mediated Epithelial Remodeling Downstream of Hedgehog-Gli Is Required for Tracheoesophageal SeparationDevelopmental cellDecember 16, 2019Nasr T, Mancini P, Rankin SA, Edwards NA, Agricola ZN, Kenny AP, Kinney JL, Daniels K, Vardanyan J, Han L, et al.Mix,Thermo Fisher Scientific,F531 Phusion Hot Start Flex 2X Master Mix,"New England BioLabs, Incorporated",M0536S QuickLoad 2X Master Mix,"New England BioLabs, Incorporated",M0271L I-Sce1,New England Biolabs,R0694S Doxycycline hyclate

(10) An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection RegimeFrontiers in Plant ScienceFebruary 26, 2019Bosibori Bett, Stephanie Gollasch, Andy Moore, Robert Harding, Thomas J. V. Higgins(IAA) Sigma-Aldrich, St. Louis, MO, United States – – 0.1 mg (35) Phusion Hot Start Flex 2X master mix (Cat # M0536S, New England Biolabs) (36) Vip3Ba forward and reverse primers: 50 GAACGCTCA GCTCAACTCCA30 and 50 GGTGGAGTTGATGAG CACGT30 (37)

(11) Comparative genome-wide characterization leading to simple sequence repeat marker development for NicotianaBMC GenomicsDecember 1, 2018Xuewen Wang, Shuai Yang, Yongdui Chen, Shumeng Zhang, Qingshi Zhao, Meng Li, Yulong Gao, Long Yang, Jeffrey L. Bennetzenforward primer, 0.53 μM reverse primer, and 10 μl Phusion Hot Start Flex 2X Master Mix (NEB, Cat. No. M0536S). For 24 markers used in further fragment scoring, a tailed forward primer was synthesized with tail

(12) Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive PluripotencyCell SystemsNovember 28, 2018Aleksandra PÄ™kowska, Bernd Klaus, Wanqing Xiang, Jacqueline Severino, Nathalie Daigle, Felix A. Klein, MaÅ‚gorzata OleÅ›, Rafael Casellas, Jan Ellenberg, Lars M. Steinmetz, et al.,New England Biolabs,M0201S T4 DNA polymerase,New England Biolabs,M0203S Phusion 2x master mix,New England Biolabs,M0536S EZlink Iodoacetyl-PEG2-Biotin,Thermo Fisher,21334 NEXTFlex DNA Barcodes,Bioo Scientific,NOVA-514102 "2′-Deoxyguanosine-5′-O-(1-thiotriphosphate)

(13) GMATA: An Integrated Software Package for Genome-Scale SSR Mining, Marker Development and ViewingFrontiers in Plant ScienceSeptember 13, 2016Xuewen Wang, Le Wangprimer, and 10 μl Phusion Hot Start Flex 2 × Master Mix (New England BioLabs, Ipswich, MA, USA, Cat. No. M0536S). PCR amplification and amplicon scoring were conducted according to our previous description for UGSW

(14) Droplet barcoding for massively parallel single-molecule deep sequencingNature CommunicationsSeptember 1, 2016Freeman Lan, John R. Haliburton, Aaron Yuan, Adam R. Abate(see oligonucleotides listed in Supplementary Table 1) using 1 × NEB phusion hotstart flex mix (NEB: M0536S) with the following cycling conditions: 98 °C 3 min, 35 cycles of: 98 °C 15 s, 62 °C 30 s, 72 °C 3 min,

(15) RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH)Visualizing RNA Dynamics in the CellJune 1, 2016Jeffrey R. Moffitt, Xiaowei ZhuangManuscript 1. 20X EvaGreen (Biotium; 31000) 2. 2X Phusion hot start polymerase master mix (New England Biolabs; M0536S) 3. Tris-EDTA (TE) pH 8 buffer (Ambion; AM9849) 4. DNA binding buffer (Zymo Research; D4004-1-L) 5. DNA

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3/31/2023Best Results with Phusion® Hot Start Flex 2X Master Mix, Minimum Optimization Soheil TavakolpourThis master mix enables researcher to minimize the time for optimization of PCR reactions, It helps to start PCR with high Tm and minimize the non-specific products.

Phusion Hot Start Flex 2X Master Mix

Phusion Hot Start Flex 2X Master Mix from New England Biolabs

New England Biolabs

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Phusion Hot Start Flex 2X Master Mix from New England Biolabs

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