Phusion Hot Start Flex 2X Master Mix M0536S from New England Biolabs

Product Specs
ItemPhusion Hot Start Flex 2X Master Mix
CompanyNew England Biolabs
Price $227.00 Inquire
Catalog NumberM0536S
Size100 reactions
ApplicationsDNA Analysis
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) DNA diamond formulates a decomposable composite letter constellation model for DNA data storageNature CommunicationsJanuary 31, 2026Qi Ge, Menghui Ren, Tingting Qi, Changcai Han, Yingjin Yuan, Weigang Chenusing Phusion High-Fidelity DNA Polymerase (Hot Start Flex 2X Master Mix, New England Biolabs, Cat# M0536S) (Supplementary Table 12). The PCR program included an initial denaturation at 98 °C for 45 s, followed

(2) Microbial metabolite 5-formamidoimidazole-4-carboxamide ribotide targets METTL1 to inhibit m7G modification of BRCA1 mRNA to inhibit high-grade serous ovarian cancerMolecular MedicineDecember 22, 2025Lixing Chen, Sili HeS rRNA sequence was PCR-amplified from the purified DNA using Phusion® Hot Start Flex 2×Master Mix (M0536S, NEB, USA). The forward primer used was 341 F (5’-CTACGGGNGGCWGCAG-3’) and the reverse primer was 805R

(3) The type 6 secretion system immunity protein RhsFI has been repurposed for small RNA regulation in pathogenic Escherichia coliNucleic Acids ResJuly 19, 2025Thomas Zammit, Brandon Sy, Vadim Shchepachev, Daniel G. Mediati, Pranita Poudyal, Dougall Norris, Samuel Bang, Saleh Alquethamy, Winton Wu, Asim Ullah, et al.(5′-CAGACGTGTGCTCTTCCGATCT-3′). Libraries were amplified using Phusion Hot Start Flex 2x Master Mix (NEB M0536S) containing 1.25 μM P5 5′oligo and barcoded 3′ reverse oligos in a 25 μl of reaction. PCR was run with

(4) Protocol for assessing regulatory elements in murine heart using an AAV9-based massively parallel reporter assaySTAR ProtocApril 17, 2025Kaiyu Wang, Meng Meng, Minghong Leng, Yanqing Wang, Jie Ni, Haiyun Yuan, Xiaoran Zhang, Yangpo Cao,Life Technologies,10977-023 TE buffer,Teknova,T0225 Phusion Hot Start Flex 2× Master Mix,New England Biolabs,M0536S Ethyl alcohol,Sigma-Aldrich,E7023-500ML Buffer EB,QIAGEN,19086 Gel loading dye,New England Biolabs,B7025S SeaKem

(5) Analysis of fungal diversity in the feces of Arborophila rufipectusFrontiers in Veterinary ScienceOctober 14, 2024Xiaoping Ma, Junshu Li, Zhiguo Li, Benping Chen, Zhenwen Ling, Shenglin Feng, Zhijun Zhong, Guangneng Peng, Ya Wang, Yaozhang Jiang, et al.performed using 2.5 μL of the reverse and forward primers, 12.5 μL of Phusion Hot Start Flex 2X Master Mix (M0536S; NEB, Beijing, China), 25 ng of template DNA, and PCR-grade water was added to make a final volume of

(6) Development and IND-enabling studies of a novel Cas9 genome-edited autologous CD34+ cell therapy to induce fetal hemoglobin for sickle cell diseaseMolecular TherapyOctober 2, 2024Varun Katta, Kiera O’Keefe, Yichao Li, Thiyagaraj Mayuranathan, Cicera R. Lazzarotto, Rachael K. Wood, Rachel M. Levine, Alicia Powers, Kalin Mayberry, Garret Manquen, et al.overhang (Table S2). PCR amplifications were performed using phusion hot start flex 2× master mix (NEB, M0536S) and 20–100 ng genomic DNA with the following conditions for HBG-sgRNA target (denaturation, 98°C for

(7) Yeast cell responses and survival during periodic osmotic stress are controlled by glucose availabilityeLifeApril 3, 2024Fabien Duveau, Céline Cordier, Lionel Chiron, Matthias Le Bec, Sylvain Pouzet, Julie Séguin, Artémis Llamosi, Benoit Sorre, Jean-Marc Di Meglio, Pascal HersenThe DNA repair fragment was obtained by PCR amplification (Phusion Hot Start Flex 2X Master Mix, NEB M0536S) of two oligonucleotides with 20 bases of reverse complementarity to each other at the 3’ end and 70

(8) Optimized single-cell RNA sequencing protocol to study early genome activation in mammalian preimplantation developmentSTAR ProtocolsSeptember 1, 2023Nina Boskovic, Gamze Yazgeldi, Sini Ezer, Mari H. Tervaniemi, Jose Inzunza, Spyridon Panagiotis Deligiannis, Barış Yaşar, Tiina Skoog, Kaarel Krjutškov, Shintaro Katayama, et al.10×",NEB,Cat# B6059S "Klenow exo, 5 U/μL",NEB,Cat# M0212S Phusion Hot Start Flex 2× Master Mix,NEB,Cat# M0536S "T4 DNA ligase, 5 U/μL",Thermo Scientific,Cat# EL0014 Terra PCR Direct Polymerase Mix,Takara,Cat#

(9) Repurposing CRISPR/Cas to Discover SARS‐CoV‐2 Detecting and Neutralizing AptamersAdvanced ScienceMay 19, 2023Ju Zhang, Airu Zhu, Miao Mei, Jing Qu, Yalan Huang, Yongshi Shi, Meiying Xue, Jingfang Zhang, Renli Zhang, Bing Zhou, et al.byproducts. The high‐fidelity polymerase Phusion Hot Start Flex 2 × Master Mix (NEB, Ipswich, USA, M0536S) was used for PCR. Then the PCR reaction was run on a 2% agarose gel, and product ≈140 bp was purified

(10) Achieving single nucleotide sensitivity in direct hybridization genome imagingNature CommunicationsDecember 15, 2022Yanbo Wang, W. Taylor Cottle, Haobo Wang, Momcilo Gavrilov, Roger S. Zou, Minh-Tam Pham, Srinivasan Yegnasubramanian, Scott Bailey, Taekjip Hasubstrates for in vitro cleavage assays were synthesized using Phusion® Hot Start Flex 2X Master Mix (NEB, M0536S) and purified using GeneJET PCR Purification Kit (Thermo Scientific, K0701). The primers were purchase

(11) Atypical cyclic di-AMP signaling is essential for Porphyromonas gingivalis growth and regulation of cell envelope homeostasis and virulencenpj Biofilms and MicrobiomesDecember 1, 2022M. Fata Moradali, Shirin Ghods, Heike Bähre, Richard J. Lamont, David A. Scott, Roland SeifertTable 2. The PCR was performed using Phusion Hot Start Flex 2X Master Mix (New England BioLabs; cat# M0536S) and consisted of 25 cycles with a temperature profile of 30 s at 98 °C, 30 seconds at 57 °C for annealing,

(12) The timing of differentiation and potency of CD8 effector function is set by RNA binding proteinsNature CommunicationsDecember 1, 2022Georg Petkau et al.PCR amplification was conducted using P5 and P3 solexa primers [Illumina] and Phusion HF master mix [NEB M0536S]. PCR products were run on 6% TBE gel and DNA in the range of 145-400 nt isolated using standard methodology.

(13) In vivo mitochondrial base editing via adeno-associated viral delivery to mouse post-mitotic tissueNature CommunicationsDecember 1, 2022Pedro Silva-Pinheiro, Pavel A. Nash, Lindsey Van Haute, Christian D. Mutti, Keira Turner, Michal Minczukindex and sequencing primers, in a 25 µl reaction, with Phusion® Hot Start Flex 2X Master Mix (NEB, M0536S) using the following primers: Nd3adapter_Fw: 5’– TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG TTC TGA

(14) Genome Oligopaint via Local Denaturation Fluorescence in Situ Hybridization (GOLD FISH)Molecular CellApril 1, 2021Yanbo Wang, Wayne Taylor Cottle, Haobo Wang, Xinyu Ashlee Feng, John Mallon, Momcilo Gavrilov, Scott Bailey, Taekjip Ha(Twist Bioscience) was amplified to a dsDNA pool using Phusion® Hot Start Flex 2X Master Mix (NEB, M0536S) by limit-cycle PCR (no more than 10 cycles). The crRNAs for ChrX ‘paint’ was in vitro transcribed using

(15) Binary Fate Choice between Closely Related Interneuronal Types Is Determined by a Fezf1-Dependent Postmitotic Transcriptional SwitchNeuronFebruary 1, 2020Yi-Rong Peng, Rebecca E. James, Wenjun Yan, Jeremy N. Kay, Alex L. Kolodkin, Joshua R. SanesNEBuilder® HiFi DNA Assembly Cloning Kit,New England BioLabs,Cat# E5520S Phusion® Hot Start Flex 2X Master Mix,New England BioLabs,Cat# M0536S SuperScript™ III First-Strand Synthesis SuperMix,ThermoFisher,Cat# 18080400 TRIzol™ Plus RNA Purification Kit

(16) Endosome-Mediated Epithelial Remodeling Downstream of Hedgehog-Gli Is Required for Tracheoesophageal SeparationDevelopmental cellDecember 16, 2019Nasr T, Mancini P, Rankin SA, Edwards NA, Agricola ZN, Kenny AP, Kinney JL, Daniels K, Vardanyan J, Han L, et al.Mix,Thermo Fisher Scientific,F531 Phusion Hot Start Flex 2X Master Mix,"New England BioLabs, Incorporated",M0536S QuickLoad 2X Master Mix,"New England BioLabs, Incorporated",M0271L I-Sce1,New England Biolabs,R0694S Doxycycline hyclate

(17) An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection RegimeFrontiers in Plant ScienceFebruary 26, 2019Bosibori Bett, Stephanie Gollasch, Andy Moore, Robert Harding, Thomas J. V. Higgins(IAA) Sigma-Aldrich, St. Louis, MO, United States – – 0.1 mg (35) Phusion Hot Start Flex 2X master mix (Cat # M0536S, New England Biolabs) (36) Vip3Ba forward and reverse primers: 50 GAACGCTCA GCTCAACTCCA30 and 50 GGTGGAGTTGATGAG CACGT30 (37)

(18) Comparative genome-wide characterization leading to simple sequence repeat marker development for NicotianaBMC GenomicsDecember 1, 2018Xuewen Wang, Shuai Yang, Yongdui Chen, Shumeng Zhang, Qingshi Zhao, Meng Li, Yulong Gao, Long Yang, Jeffrey L. Bennetzenforward primer, 0.53 μM reverse primer, and 10 μl Phusion Hot Start Flex 2X Master Mix (NEB, Cat. No. M0536S). For 24 markers used in further fragment scoring, a tailed forward primer was synthesized with tail

(19) Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive PluripotencyCell systemsNovember 28, 2018Pękowska A, Klaus B, Xiang W, Severino J, Daigle N, Klein FA, Oleś M, Casellas R, Ellenberg J, Steinmetz LM, et al.,New England Biolabs,M0201S T4 DNA polymerase,New England Biolabs,M0203S Phusion 2x master mix,New England Biolabs,M0536S EZlink Iodoacetyl-PEG2-Biotin,Thermo Fisher,21334 NEXTFlex DNA Barcodes,Bioo Scientific,NOVA-514102 "2′-Deoxyguanosine-5′-O-(1-thiotriphosphate)

(20) GMATA: An Integrated Software Package for Genome-Scale SSR Mining, Marker Development and ViewingFrontiers in Plant ScienceSeptember 13, 2016Xuewen Wang, Le Wangprimer, and 10 μl Phusion Hot Start Flex 2 × Master Mix (New England BioLabs, Ipswich, MA, USA, Cat. No. M0536S). PCR amplification and amplicon scoring were conducted according to our previous description for UGSW

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3/31/2023Best Results with Phusion® Hot Start Flex 2X Master Mix, Minimum Optimization Soheil TavakolpourThis master mix enables researcher to minimize the time for optimization of PCR reactions, It helps to start PCR with high Tm and minimize the non-specific products.

Phusion Hot Start Flex 2X Master Mix

Phusion Hot Start Flex 2X Master Mix from New England Biolabs

New England Biolabs

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Phusion Hot Start Flex 2X Master Mix from New England Biolabs

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