Laemmli Sample Buffer Works Well for Immediate Western Blotting

Penn State College of Medicine
Neurosurgery
Research Intern

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

2x Laemmli Sample Buffer

Catalog Number:

1610737

I often study cells grown in bi-chamber plates, leading to much difficultly in collecting them for western blotting. In those circumstances, I use this with BME to denature and reduce the cells right on the inserts. This allows me to collect all of the cells and directly load for western blotting.

Experimental Design and Results Summary

Application

Western blot

Starting Material

N/A

Tips

No Notes

Results Summary

I was this for all of my insert cells for western blotting. I can nicely collect the cells after they are denatured with this and the BME added.

DOI or PMID #

N/A

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Summary

The Good

Great for direct cell denaturing.

The Bad

Smells when BME is added.

The Bottom Line

I recommend.

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