BioLegend
421™ anti-mouse CD138 (Syndecan-1) Antibody
142507
281-2
Info:Supplier Page View Product Specs
Browse Similar Products: CD138 Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are looking for markers of PC, and for that we stain PCs using CD138.
Flow Cytometry
Mouse spleen
Surface staining with CD138 BV421 (1/800), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer with a violet laser.
As it can be observed in the figure, plasma cells (PCs) can clear be stained among singlets and lymphocytes. The frequency of PCs in the spleen increase with age of the mice, but is around 0.3-1% in adult mice.
30005826, 33479892, 35464469
Reliable and good signal.
Nothing really.
This is the best option in the marker in my opinion.