Key Reagent in the SDS Lysis Buffer

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

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Company:

Invitrogen™

Product Name:

UltraPure™ SDS Solution, 10%

Catalog Number:

24-730-020

We use UltraPure 10% SDS (sodium dodecyl sulfate) Solution when making the SDS lysis buffer, a buffer solution used for the purpose of breaking open cells (SDS is able to disrupt cell membranes) and get the lysate for western blot purpose.

Experimental Design and Results Summary

Application

SDS lysis buffer

Starting Material

Cell culture

Tips

N/A

Results Summary

We use UltraPure 10% SDS (sodium dodecyl sulfate) Solution when making the HOT SDS lysis buffer, a buffer solution used for the purpose of breaking open cells. We prefer this buffer when we want to get nuclear protein extraction from our cells.

DOI or PMID #

N/A

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Summary

The Good

Effectively extracting nuclear proteins.

The Bad

N/A

The Bottom Line

SDS is a strong ionic agent able to disrupt cell membranes and get the lysate for western blot purpose.

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