sAPP-Alpha (2B3) Anti-Human Mouse IgG MoAb Probably Worked Technically

February 14, 2023

BlueskyReddit
Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

IBL- America

Product Name:

sAPP-alpha antibody

Catalog Number:

11088

Clone Number:

N/A

I tried to use this to determine if my cells secreted sAPP-alpha in different amounts in response to a stimulus. I concentrated the cell media and then ran for western blotting. I did not see a clear band, but instead a smear at the top. I think the antibody worked, but the concentrated media did not allow the proteins to properly run through the gel.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human astrocytes

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

I got better results using an ELISA from the same company. But I think the antibody would work if the gel ran properly.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Probably works.

The Bad

ELISA is better.

The Bottom Line

I wouldn't not recommend.

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