Invitrogen
HA-tag Antibody
MA5-27915
N/A
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I tried to use this antibody to verify the coIP of my HA-tagged protein. I saw 'bands' but not at my protein molecular weight an nothing in the cell lysate. After reflection, I concluded that the bands were heavy chain IgG from the magnetic beads used in the pull down. When I repeated the experiments and probed with a different HA-tag antibody, I saw the bands I was looking for.
Western Blot
HEK 293 cells
1:1000 overnight BSA
BSA
1 hr, milk
ECL
This did not work for me to detect hA-tagged ferroportin. I wasted months of coIP trouble shooting because I didn't see my bands. Using a different antibody in western blotting would have saved me so much time and sanity. The blot shows my failed experiment. The first lane (IP) is a cross reaction of the secondary antibody with the heavy chain IgG from the magnetic beads. The other lane is the whole cell lysate.
Nothing.
Does not work.
Would not recommend.