Activation mTOR Pathway for WB

December 28, 2022

BlueskyReddit
Houston Methodist Research Institute

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Company:

Cell Signaling Technology

Product Name:

Phospho-mTOR (Ser2481) Antibody

Catalog Number:

2974S

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: MTOR Antibodies

Cells (fibroblast, MRC-5 human cell lines) were starved form 4 hours (0,5% FBS) and induced with TGF-b. eIF4E siRNA was transfected with lipofectamine RNAmax for 24 hours.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Cells (fibroblast, MRC-5 human cell lines) were starved form 4 hours (0,5% FBS) and induced with TGF-b. eIF4E siRNA was transfected with lipofectamine RNAmax for 24 hours.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Recommended.

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